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Biological Buffers

Biological Buffers

Biological buffers are inert and have short-term effects on the system under investigation, both of which are distinct advantage over inorganic substances and weak organic acids that were used in the past. Most biological buffers in use today are N-substituted taurine or glycine buffers, which meet most of the requirements that biological buffers have to fulfill. Buffer systems are usually used for experiments to enable direct comparison of results.

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Phosphate Buffer Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Contains sodium and potassium phosphates. Intended for use in the Sigma Butyrate Esterase Procedure, No. 181B.

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Citrate Buffer, Concentrate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer solution, 0.38 mol/L, pH 5.4 when diluted according to procedure 386A.

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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Buffer concentrate, citrate phosphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.

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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology

Supplier: VWR Chemicals

Sodium acetate buffer, VWR®, 3 M, pH value: 5,2

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VWR® A.C.E.™, Sequencing buffers

VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

J.T.Baker® DILUT-IT™, Potassium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

Supplier: VWR Chemicals

TRIS buffer solution pH 7,5 (1 mol/l), VWR®

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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

Supplier: VWR Chemicals

HEPES buffer solution pH 7.3 (1 mol/l), VWR®

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Optimizer Buffer™ for optimal cross-linking and modification

Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.

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Buffer kit for calibration of fluorescent Ca² indicators

Supplier: Biotium

Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).

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RNA isolation buffers

Supplier: OMEGA BIO-TEK

RNA Wash Buffer II concentrate

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Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Buffers, TRIS HCl solutions, pH range 7,2 to 8,5 (1 mol/L)

Supplier: Rockland Immunochemicals

Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.

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EGTA (2X) Buffer, Enzo Life Sciences

Supplier: Enzo Life Sciences

EGTA buffer, 2X

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Neutralising reagent

Supplier: Enzo Life Sciences

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

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Buffer, HEPES solution (1 mol/L)

Supplier: Cayman Chemical

HEPES buffer solution (1 mol/l)

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