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57 results for Vectors, Plasmids and Libraries

 

Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

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Human Tagged ORF Clone, SLC52A3

Supplier: OriGene

Lenti ORF particles, SLC52A3 (mGFP-tagged)-Human chromosome 20 open reading frame 54 (C20orf54), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, GPR172B (SLC52A1)

Supplier: OriGene

Lenti ORF particles, SLC52A1 (mGFP-tagged) - Human G protein-coupled receptor 172B (GPR172B), transcript variant 1, 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, SGOL2 (SGO2)

Supplier: OriGene

SGOL2 (Myc-DDK-tagged)-Human shugoshin-like 2 (S. pombe) (SGOL2), transcript variant 1. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, CFTR (NM_000492)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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InterPlay Adenoviral TAP Systems

Supplier: AGILENT

The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

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AAV helper-free system

Supplier: AGILENT

The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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Human Tagged ORF Clone, GPCR MRGX2 (MRGPRX2)

Supplier: OriGene

MRGPRX2 (tGFP-tagged) - Human MAS-related GPR, member X2 (MRGPRX2). This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone, P Glycoprotein (ABCB1)

Supplier: OriGene

This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. ABCB1 (tGFP-tagged) - Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1).

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Human Tagged ORF Clone, GPR172A (SLC52A2)

Supplier: OriGene

Lenti ORF particles, SLC52A2 (mGFP-tagged) - Human G protein-coupled receptor 172A (GPR172A), 200 µl, >10⁷ TU/ml. This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Tagged ORF Clone Lentiviral Particle, PD-L1 (CD274)

Supplier: OriGene

Lenti ORF particles, CD274 (Myc-DDK tagged) - Human CD274 molecule (CD274), 200 µl, >10⁷ TU/ml.

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AdEasy Adenoviral vector systems

Supplier: AGILENT

The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

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Vectors, pBlueScript II

Supplier: AGILENT

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

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SureVector

SureVector

Supplier: AGILENT

Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

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Human cDNA Clone

Supplier: OriGene

KCNH2 (untagged) - human potassium voltage-gated channel, subfamily H (eag-related), member 2 (KCNH2), transcript variant 3

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Human Untagged LRRC15 Clone

Supplier: OriGene

LRRC15 (untagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2.

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pGFP-C-shLenti shRNA Vector

Supplier: OriGene

pGFP-C-shLenti vector is a third generation Lentivector which requires the viral components carried in other vectors to produce viral particles. There are three major functional elements within the 5LTR and 3LTR: a shRNA expression cassette driven by an U6 promoter, a puromycin resistant gene driven by a SV40 promoter and a tGFP gene driven by a CMV promoter. All of them can be packaged to viral particles and transduced to many cell lines. The bacterial selection marker for the vector is Chloramphenicol.

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P2ry2 (NM_008773) Mouse Tagged ORF Clone

Supplier: OriGene

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use.

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Lambda DNA

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Lambda DNA is a temperate E. coli bacteriophage.

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pAAV-AC-GFP AAV Gene Expression Vector

Supplier: OriGene

AAV Gene Expression Vectors with C-terminal tGFP tag.

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Mammalian TAP system, interPlay

Supplier: AGILENT

The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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Mouse Untagged Clone Plasmid, Lrrc15 (NM_028973)

Supplier: OriGene

Lrrc15 (untagged) - Mouse leucine rich repeat containing 15 (Lrrc15).

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ORF Clones, GABA B receptor 2 (GABBR2), human tagged, TrueORF Gold

Supplier: OriGene

GABA B receptor 2 (GABBR2), human tagged ORF clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

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Human Untagged FBXL17 Clone

Supplier: OriGene

FBXL17 (untagged)-Human F-box and leucine-rich repeat protein 17 (FBXL17). The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10 µg of transfection-ready, dried plasmid DNA (reconstitute with 100 µl of water).

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Human shRNA plasmid kit, ADHFE1

Supplier: OriGene

Human shRNA plasmid kit, ADHFE1 is designed against multiple splice variants at this gene locus.

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Lentiviral Control Particles

Supplier: OriGene

Lenti particles carrying a 29-mer scrambled sequence cassette and serve as a negative control.

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AAV1/2-CMV/CBA-Human-A53T-alpha-SYN AAV Vector

Supplier: Charles River Laboratories Cell Solutions

AAV1/2 Vector with CMV promoter, human A53T alpha-synuclein, WPRE, bovine growth hormone (BGH), and polyadenylation sequence.

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