Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of hepatitis viruses.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of human herpes viruses.

Supplier: PRIMERDESIGN

Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. Although more than 100 different human papillomavirus (HPV) types have been characterised HPV 6, HPV 11, HPV-16 and HPV 18 are the most clinically relevant.

Supplier: BIOPREMIER

SUPREME Real time detection uses real-time PCR for the detection of DNA present in the mitochondrial genome in a simple, reliable, and rapid procedure. The assay is based on 5’ nuclease real time PCR reactions to amplify a unique genomic sequence in the target.

Supplier: PRIMERDESIGN

PCR kits for the detection and simultaneous quantification of gastrointestinal pathogens.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of a specific pathogenic bacteria. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target microorganism. PCR is a method used to amplify a specific DNA sequence which is typically amplified in a reaction containing a thermostable DNA polymerase, nucleotides, and primers complementary to the target sequence. When this solution is heated, the DNA molecule denatures, separating into two strands. As the solution cools, the primers anneal to the target sequences in the separated DNA strands and the DNA polymerase synthesises a new strand by extending the primers with nucleotides, creating a copy of the DNA sequence (amplicons). When repeated, this cycle of denaturing, annealing, and extending exponentially increases the number of target amplicons. In Real-Time PCR, specific fluorescent probes are used to detect the amplified DNA by hybridising with amplicons. These probes are linked to a fluorophore on one end and a quencher that suppresses fluorescence on the other. If the target sequence is present during the PCR, amplification occurs and the probe is degraded, resulting in fluorescence increase. Fluorescence is measured by a detector and the associated software plots the fluorescence intensity versus number of cycles, allowing the determination of the presence or absence of the target organism.

Supplier: HYSERVE

To detect allergen in highly processed foods by special extraction solution and unique antibody. This lateral-flow test is a high-performance qualitative assay.

Supplier: Sartorius

The Microsart® ATMP Mycoplasma detection kit is especially designed for an easy and fast detection of mycoplasma in cell-based therapeutics like autologous chondrocyte transplants (ATMPs –advanced therapy medical products). Due to the short shelf life of these therapeutics, a fast result is needed. The PCR-technology makes it possible to reduce the time-to-result to only 3 hours.

Supplier: BIOPREMIER

These kits allow the detection of allergic substances. Ideal for controlling and monitoring the various steps of food production.

Supplier: Genscript

ToxinSensor™ Gel Clot Endotoxin Assay Kit is intended as an in vitro end-product endotoxin test for human and animal parenteral drugs, biological products, and medical devices. The limulus amebocyte lysate (LAL) test is a qualitative test for Gram-negative bacterial endotoxin.

Supplier: Genscript

ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit is designed to be a quantitative In vitro end-point endotoxin test for human and animal parenteral drugs, biological products, and medical devices.

Supplier: MACHEREY-NAGEL

These products are used in testing the resistance of pathogens to antibiotics. For this test the filter paper sections can be impregnated with the antibiotic to be tested and placed on the inoculated nutrient medium. Depending on the effectiveness, a smaller or larger zone of inhibition is formed.

Supplier: BIOPREMIER

Gluten detection test kit by using qPCR. Celiac disease is an autoimmune disorder associated with an intolerance to gluten proteins mainly present in wheat, barley, and rye.

Supplier: BIOPREMIER

Food safe kits provide a simple, reliable, and rapid procedure for detecting the presence of an animal species in meat and meat products. The assay is based on 5’ nuclease Real-Time PCR reactions to amplify a unique genomic sequence in the target organism. According to European Commission Directive 2002/86/EC, food ingredients have to be declared. In the case of meat and meat products, the animal species has to be disclosed on the label. Moreover, the species autenticity can be highly relevant to consumers for economic, medical, cultural and religious reasons. Fraudulent substitution of cheaper meats in place of more expensive species, the inclusion of meat in non meat (vegetarian) products and the presence of allergens in food products are clear examples for the importance of this issue (Ballin, 2010; Dooley et al., 2004). Several methods are based on identification of proteins by means of electrophoretic and/or immunological methods. However, these methods are not reliable for application in highly processed and heated products due to the protein deterioration. DNA is more stable than proteins during processing and although it can be fragmented by several processes, modern DNA methodologies like PCR-based techniques still allow the identification of DNA from the different species present in a sample (Lockley & Bardsley, 2000). Real-Time PCR techniques are especially suitable for these products because small fragments of DNA can still be amplified and identified with high sensitivity and specificity (Dooley et al., 2004).

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

Successful PCR applications depend on well purified DNA. In diagnostics and analytics of food and medical samples PCR and real-time PCR has many advantages over conventional methods. In all cases it is of importance to have well purified DNA for subsequent PCR, real-time PCR, hybridization or sequencing purposes. Purified DNA leads to higher sensitivity and also specificity of PCR based tests in difficult matrices. With QuickBlue Extraction and Purification Kit you always get high DNA yields and highly purified DNA. The kit uses paramagnetic nano-particles for purification. It can help to purify DNA from difficult matrices like food or even blood in soil for applications in forensic sciences. DNA in Seawater can also be purified. This method avoids extensive centrifugation steps and can thus be applied in environments where centrifuges are not usable. The kits includes proteinase K as a reagent for cell disruption, so that extraction and purification can be performed with the kit. DNA yield from 1 ml bacterial enrichment is 10 μg of Genomic DNA. The protocol can be adapted to automated magnetic bead separation instruments.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. It also includes a test for Shigella. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and you avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Listeria monocytogenes in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838.Test principle. The test is dedicated to detect DNA of Listeria monocytogenes in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

Vibrio vulnificus causes severe wound infections and is harmful when ingested. 33% of patients being infected die. The detection in food and water samples using this Real-Time PCR Test is very rapid and versatile. You have results available already after 24 hours. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research “VibrioNet” with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-50. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Hazel-nut is allergenic for a considerable number of individuals. Using DNA based tests also processed food samples might be tested. Our Real-Time PCR test exhibits the required limit of detection laid down in the codex alimentarius. The Real-Time PCR kit has a limit of detection of ≤ 20 DNA-copies. The limit of detection can be influenced by sample matrix, level of processing, DNA-preparation and DNA-content.

Supplier: Q BIOANALYTIC

Pseudomonas aeruginosa is tested usually with cultural methods. However, this may take several days to deliver results. Using this Real-Time PCR test you may have results already after a few hours using a DNA purification directly from filtered water without pre-enrichment. You can also use this test in combination with an appropriate pre- enrichment according to DIN EN ISO 20837. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae . With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research 'VibrioNet' with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-19. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Vibrio parahaemolyticus tox gene (tdh/trh): Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for Vibrio parahaemolyticus for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: ROSCO

Antibiotic Tests, KPC/MBLl Oxa

Supplier: Q BIOANALYTIC

Real-Time kit for the detection of Staphylococcus aureus.

Supplier: ROSCO

Diatabs offers an accurate and rapid identification of clinically relevant bacteria and yeasts. The concept of individual tests - used as single tests or in combination - offers the user many advantages over traditional methods. The Diatabs system offers the opportunity of finding the most appropriate and economical tests for a number of purposes.