You searched for: Protein Purification

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

Supplier: MP Biomedicals

Protein A is a recombinant Fc-binding protein from Staphylococcus aureus, Cowan strain-1 gene. The product is a carboxy truncated material containing 301 amino acid residues. The non-binding regions have been removed to minimise potenial steric interference.

Supplier: Cytiva

Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

Supplier: Cytiva

ANX Sepharose™ 4 Fast Flow (high sub) is a weak anion exchanger, with a different selectivity compared with traditional ion exchangers.

Supplier: Cytiva

Q Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with quaternary (Q) strong anion exchange groups.

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

Supplier: Cytiva

DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: Cytiva

Biacore maintenance kits are convenient kits for proper instrument maintenance.

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

Supplier: Merck

Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: Merck

Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Kits are designed to provide fast, simple, highly reproducible and sensitive immunocapture and protein digestion in a single well. These kits remove the challenges often associated with the process of biomarker quantitation, by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight.

Supplier: Thermo Fisher Scientific

SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Trypsin Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Proteinase K Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments. This version of the kit utilises Proteinase K, which cleaves the peptide bond adjacent to the carboxyl gropu of aliphatic and aromatic amino acids with blocked alpha amino groups.

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Human IgA Purification Kit with LT technology utilises novel, patented ligands, designed for IgA antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of human IgA in just ten easy steps.

Supplier: LIGATRAP TECHNOLOGIES

LigaTrap® Mouse IgM Purification Kit with LT technology utilises novel, patented ligands, designed for IgM antibody purification. Kit provides all the necessary reagents for fast, convenient micro-scale purification of mouse IgM in just ten easy steps.