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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

High Select™ top14 abundant protein depletion resin, Pierce™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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GlycoLink™ IP kit

GlycoLink™ IP kit

Supplier: Thermo Fisher Scientific

Thermo Scientific™ GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilisation of antibodies and other glycoproteins via oxidised sugar groups.

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Pierce™ Conjugate Purification Kit

Pierce™ Conjugate Purification Kit

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

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Classic IP kit, Pierce™

Classic IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Classic IP Kit uses protein A/G agarose beads, microcentrifuge spin columns and buffers to perform traditional immunoprecipitation assays.

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AminoLink™ Plus micro immobilisation kit, Pierce™

AminoLink™ Plus micro immobilisation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ AminoLink™ Plus Micro immobilisation kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

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c-Myc Tag IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ c-Myc Tag IP/Co-IP Kit provides a simple and fast method to study c-Myc-tagged proteins, with advantages over the traditional IP procedure using protein A/G agarose.

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Pierce™ Protein A/G Coated Plate IP Kit

Pierce™ Protein A/G Coated Plate IP Kit

Supplier: Thermo Fisher Scientific

Pierce™ protein A/G coated plate IP kit uses coated protein A/G microplates to perform immunoprecipitation assays in 96-well plates without resins, beads, centrifugation or magnets.

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High Select™ HSA/immunoglobulin depletion resin

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select HSA/Immunoglobulin Depletion Resin decreases the albumin and antibody components that are abundant in human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

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TCEP solution, neutral pH, Bond-Breaker™ Pierce™

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Pierce™ Protein A

Pierce™ Protein A

Supplier: Thermo Fisher Scientific

Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Pierce™ SDS-PAGE Sample Prep Kit

Pierce™ SDS-PAGE Sample Prep Kit

Supplier: Thermo Fisher Scientific

Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

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Streptavidin HRP conjugates, high sensitivity, Pierce™

Streptavidin HRP conjugates, high sensitivity, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ High Sensitivity Streptavidin-HRP is a peroxidase-conjugated streptavidin biotin-binding protein that provides signal amplification like poly-HRP and exceptional storage stability.

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Recombinant protein A, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Recombinant protein L, peroxidase conjugated, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

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Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Affinity purification kits, Melon™ Gel spin plate kit for IgG screening

Supplier: Thermo Fisher Scientific

The Melon™ Gel Spin Plate Kit for IgG Screening offers a high throughput format for quick purification and screening of antibodies.

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Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Maleimide, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

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F(ab')2 Micro preparation kit, Pierce™

F(ab')2 Micro preparation kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

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Fab Preparation kits, Pierce™

Fab Preparation kits, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Fab preparation kits use immobilised papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

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Inclusion body solubilisation reagent, Pierce™

Inclusion body solubilisation reagent, Pierce™

Supplier: Thermo Fisher Scientific

Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.

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Direct magnetic IP/Co-IP kit, Pierce™

Direct magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

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Crosslink magnetic IP/Co-IP kit, Pierce™

Crosslink magnetic IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilise IP antibodies onto premium-quality protein A/G magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

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3M™ Polisher ST Laboratory Capsules

3M™ Polisher ST Laboratory Capsules

Supplier: 3M

3M™ Polisher ST is a fully encapsulated anion exchange (AEX) product to reduce host sell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography of biopharmaceutical process streams.

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Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.

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Immobilised ficin (agarose resin), Pierce™

Immobilised ficin (agarose resin), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Immobilised Ficin consists of a sulfhydryl-specific protease (ficin) that has been immobilised onto beaded agarose resin to enable controlled antibody fragmentation, especially of mouse IgG1.

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Immobilised TCEP disulphide reducing gel, Pierce™

Immobilised TCEP disulphide reducing gel, Pierce™

Supplier: Thermo Fisher Scientific

Immobilised TCEP disulphide Reducing Gel eliminates the need to use laborious and inefficient gel filtration methods to separate the reduced sample from the reducing agent.

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Mouse IgG1 Fab and F(ab')2 preparation kits, Pierce™

Mouse IgG1 Fab and F(ab')2 preparation kits, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilised ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments.

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HA-Tag IP/Co-IP kit, Pierce™

HA-Tag IP/Co-IP kit, Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.

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Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Amino acid modification reagents, sulfhydryl addition kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.

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HA-Tag Magnetic IP/Co-IP Kit, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ HA-Tag Magnetic IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.

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