You searched for: Protein Purification

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

The Pierce™ Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilised from inclusion bodies.

Supplier: Thermo Fisher Scientific

UltraLink™ iodoacetyl resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblisation of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.

Supplier: Thermo Fisher Scientific

Hydrazide-activated resin to immobilise glycoproteins for large-scale affinity purification.

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

Supplier: Thermo Fisher Scientific

Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

SulfoLink™ coupling resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilisation of cysteine-peptides and other sulfhydryl molecules.

Supplier: Thermo Fisher Scientific

Pierce™ CDI-activated agarose is carbonyldiimidazole affinity chromatography resin, activated for covalent immobilisation of N-nucleophiles and primary amine ligands in aqueous or organic solvent conditions.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

Supplier: Thermo Fisher Scientific

Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.

Supplier: Thermo Fisher Scientific

The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.

Supplier: Thermo Fisher Scientific

NHS-activated agarose is an amine-reactive, beaded-agarose resin for rapid and stable immobilisation of proteins, peptides and other ligands via primary amines.

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

Supplier: Thermo Fisher Scientific

Pierce™ AminoLink™ Plus Micro immobilisation kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.

Supplier: Thermo Fisher Scientific

The Pierce™ IgM Purification Kit is an easy to use affinity matrix and buffer system to purify IgM from mouse ascites fluid. The method uses immobilised mannan binding protein (MBP), which binds to IgM in the kit-supplied buffers.

Supplier: Thermo Fisher Scientific

Immobilisation Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Supplier: Thermo Fisher Scientific

Pierce™ conjugate purification kit is designed to eliminate excess, unconjugated HRP or alkaline phosphatase enzyme from labeled antibodies (IgG) after performing antibody-enzyme conjugation reactions. The single-use column-kit is intended for purification of 0,5 to 50 mg of antibody conjugate dissolved in tris-buffered saline.

Supplier: Thermo Fisher Scientific

Pierce™ High Capacity Endotoxin Removal Resins are ideal for the removal of endotoxins from protein or antibody samples prior to cell transfection or animal injection.

Supplier: Thermo Fisher Scientific

Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.

Supplier: Thermo Fisher Scientific

Pierce™ Streptavidin agarose is a standard-capacity crosslinked 6% beaded agarose resin of immobilised recombinant streptavidin protein for use in a variety of biotin-binding affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates.

Supplier: Thermo Fisher Scientific

These beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.

Supplier: Thermo Fisher Scientific

Immobilised GST is glutathione S-transferase from Schistosoma japonicum that has been covalently immobilised on crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

Pierce™ Thiophilic adsorbent is a protein-free immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human. This Pierce™ Thiophilic Adsorption Kit is a purification kit containing columns pre-packed with the resin and ready-to-use buffers.

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ High Select Top14 Abundant Protein Depletion Resin decreases the abundant protein and antibody components of human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.

Supplier: Thermo Fisher Scientific

Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Supplier: Thermo Fisher Scientific

Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.