A protein expression system is a genetic construct designed to produce a protein. Protein expression systems are used in research and are also used to produce antibodies, vaccines, and enzymes commercially.
Protein Expression Technology, Kits and Resources
Protein expression is the process of generating a specific protein by manipulating the gene expression in a living organism. The organisms used for protein expression technology must be easy to culture and maintain, grow rapidly, and produce large amounts of protein.
Shop the Best Products for Protein Expression Technology
Avantor® carries innovative protein expression products from trusted suppliers at Avantor. Shop from a wide range of high-performance lab equipment, tools and supplies for protein expression systems. We also deliver the best values in the marketplace. Look through our portfolio of exclusive brands or work with us for a tailored approach. Avantor can help you set science in motion with our additional services. Learn more.
Learn more about our protein expression technology, kits and resources.
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Featured Suppliers
From environmental control to cleanrooms, pathogen detection in food and beverage, cosmetics, personal care and cannabis labs, Hardy provides solutions.
Seradigm’s unique collection and manufacturing techniques preserve more of the naturally occurring growth factors in sera that cells require to thrive.
Construct Generation and Introduction
Successfully introduce and incorporate genetic material into your target cell or tissue with effective protein expression technology from Avantor.
Vectors for cloning and expression.
View all the best-in-class Electroporators we offer.
A full line of Electroporation Cuvettes to meet your needs.
Increase transformation and transposition efficiencies with a high-quality Electroporation Buffer from Avantor.
Enzymes and libraries for CRISPR/Cas9
Kits to perform single and multiple site-directed mutagenesis.
Enzymes to modify nucleic acid molecules.
Broad selection of restriction enzymes to cleave DNA molecules at specific sites.
See our wide offering of Transfection Reagents.
Grow - Prokaryotic Cells (Yeast & Bacterial Cell Culture)
Find the right protein expression products to help ensure the maximum yield of prokaryotic cells.
We offer a large variety of competent cells for cloning and protein work.
Cultivate and isolate microorganisms for research purposes. Fast-dissolving powders come premixed for quick preparation using neutral liquids.
Microbiology additives and supplements enhance the growth effectiveness in base media and allow researchers to customize the media accordingly to their needs.
Agar powder for bacteriological applications.
Plated or contained in tubes to guarantee that the correct nutrient mixtures are present for specific cell types.
Shaking incubators for cell culturing, cell aeration, and solubility studies.
Grow - Eukaryotic cells (Mammalian & Insect Cell Culture)
Avantor is your source for everything your lab needs to help ensure high-volume eukaryotic cell growth.
Find the more used antibiotic mixtures for cell and tissue culture.
Recommended reagent for use as selection agent.
Multilayer cell culture chambers and bioreactors for scale-up cell culture needs.
Choose from a wide variety of origin-certified and quality-controlled sera for your general or specific cell culture applications.
See our range of defined and specialty media for mammalian & insect cells.
Select from the most common buffers used to provide an energy source, maintain proper pH levels, and keep ideal osmotic pressure.
Cell culture media supplements compensate for characteristics the base medium lacks in providing maximum cell growth conditions.
See our wide offering of Transfection Reagents.
QA/QC
Quality control is a critical step in protein expression. Shop Avantor for reliable quality assurance supplies.
Microbial air samplers with single and dual head configurations or samplers for other airborne particles like viruses, spores and others.
Protect your body from liquid spray, dry particulates, and spilled chemicals with quality clothing articles.
Extensive selection of cleaning agents for critical environments without leaving residues
Plates for environmental monitoring of surfaces and air.
Cultivate and isolate microorganisms for research purposes. Fast-dissolving powders come premixed for quick preparation using neutral liquids.
See our selection of kits to detect mycoplasma contamination in cell cultures.
What Is a Protein Expression System?
What Is the Difference Between Protein Expression and Protein Purification?
The difference between protein expression and protein purification is that protein expression is the process of producing proteins while protein purification is the process of isolating proteins of interest.
The level of purity needed corresponds to the end use of the protein. A purity of 90-95% may qualify a protein for research purposes, but proteins meant for use in pharmaceutical applications require purity levels up to 99.99%.
The purification workflow follows several steps:
- Protein is extracted from cells by lysing. ·
- A centrifuge separates soluble from insoluble components. ·
- Chromatography purifies the protein. ·
- Protein is quantified and checked for purity.
What Is the Purpose of Protein Expression?
The purpose of protein expression is to manufacture a functional protein in a laboratory that can be used for research or commercial use. Protein expression technology may be accomplished using protein expression kits designed for specific types of expression.
Various cell types can be used for protein expression, including:
- Bacteria
- Yeast
- Insect
- Mammalian
Protein expression can also be done by using a cell-free protein expression system.
Large-scale recombinant protein expression is used to produce enzymes, antibodies, or vaccines. This type of protein production requires a high volume, and the system must make it easy to culture, maintain, and grow proteins rapidly.
Featured Products
Available in Petri, Contact plate, or Compact Dry formats, 100-200L per minute models, and a variety of ergonomic, lightweight platforms that make active impact air monitoring a breeze.
Beckman Vi-Cell
Validated reagent with appropriate cleaning and disinfectant solutions. Ensures optimum performance and results from your Vi-CELL.
Hardy Fluid A
Sterility media and rinse solutions are manufactured in an ISO 13485 environmentally controlled production center. Each lot is certified for pH, sterility and growth promotion using ATCC® strains specified by the USP.
Dissociate and harvest cells with HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin.
Hardy Lok-Tight™ Contact Plates
Recommended for use in the cultivation of microorganisms from environmental surfaces. Friction lid design keeps lids in place
Avantor Seradigm Select Grade FBS provides superior performance and consistency in cost-sensitive applications. Sourced from countries with health status classified as “Negligible BSE Risk” by the World Health Organization.
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