Supplier: Cytiva

HiScreen Capto MMC ImpRes is a ready-to-use column pre-packed with BioProcess Capto MMC ImpRes weak cation exchange multimodal resin. The columns are an excellent choice for method optimization and parameter screening.

Supplier: Cytiva

Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.

Supplier: VARIAN

Alumina, like silica, is an extremely polar sorbent. The alumina surface tends to be slightly more stable under high pH conditions than unfunctionalised silica. The small particle size of the Bond Elut™ Alumina range ensures high extraction efficiency even when small bed masses are used.

Supplier: VARIAN

Bond Elut™ ENV, a PS/DVB polymer, is designed for the extraction of polar organic residues. It contains 125 μm spherical particles with a high degree of cross linking, advantageous for high volume, fast flow-through applications.

Supplier: Cytiva

HiScreen™ MabSelect Xtra™ is prepacked with MabSelect Xtra™ of 4,7 ml volume and bed height of 10 cm make HiScreen™ columns excellent tools for method optimisation, parameter screening for capture of monoclonal antibodies (MAbs), robustness testing, and convenient scale-up.

Supplier: Cytiva

HiScreen Capto adhere ImpRes is a ready-to-use column pre-packed with BioProcess Capto adhere ImpRes strong anion exchange multimodal resin. The columns are an excellent choice for method optimization and parameter screening.

Supplier: Cytiva

GSTPrep™ FF 16/10 is prepacked, ready-to-use column containing glutathione Sepharose™ 4 fast flow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.

Supplier: Cytiva

HiScreen Q FF columns are prepacked with Q Sepharose Fast Flow, a strong anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Supplier: Cytiva

HiScreen™ SP HP columns are prepacked with SP Sepharose™ High Performance ion exchange chromatography media of 4,7 ml volume and bed height of 10 cm make HiScreen™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up.

Supplier: Agilent

High quality SampliQ SPE (Solid Phase Extraction) products help the user confidently extract and concentrate samples from complex matrices, ensuring fast, accurate, and reproducible results from the very first step. The SampliQ line includes polymers, silica and non-silica options to meet user's specific SPE needs.

Supplier: Cytiva

MAbTrap™ is an affinity chromatography kit for fast and effective purification of monoclonal and polyclonal IgG from ascites fluid, serum and cell culture supernatants. MAbTrap™ Kit contains everything necessary to perform a complete one step purification without using complicated equipment. It also eliminates time consuming buffer preparation and column packing.

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.Two levels of substitution help find optimal selectivity and binding capacity for a given application.

Supplier: Cytiva

Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.Two levels of substitution help find optimal selectivity and binding capacity for a given application.

Supplier: VARIAN

A medium polarity sorbent with many uses, Bond Elut™ CN-E is ideal for applications in which extremely non-polar compounds would be irreversibly retained on high carbon load sorbents such as C₈ and C₁₈. This endcapped version of the cyano sorbent is best utilised when extracting analytes from an aqueous matrix.

Supplier: VARIAN

Bond Elut™ CH is a mid-polarity sorbent that exhibits unique selectivities for certain analytes. When employed as a non-polar sorbent, CH has the approximate polarity of a C₂ sorbent. Bond Elut™ CH is often a good choice when non-polar sorbents such as C₁₈, C₈ or C₂ do not provide the desired selectivity.

Supplier: DWK Life Sciences

These borosilicate glass gravity columns are manufactured from medium wall tubing with PTFE stopcock. These columns have a coarse porosity glass frit (40 - 60 μm pore size) at the lower end to act as a support for column packing material. Also these columns can be used alone or in combination with reservoirs and flow control adapters.

Supplier: TOSOH Bioscience

SkillPak 50 and 200 ml columns are designed for fast method development and scale-up and are pre-packed with TOYOPEARL® and TSKgel® process chromatography media. These columns offer superior separation of biomelocules, such as monoclonal antibodies, proteins and oligonucleotides. They are the best solution to scale up methods developed on SkillPak 1 and 5 ml pre-packed columns.

Supplier: VARIAN

Bond Elut AccuCAT cartridges are mixed bed SPE cartridges consisting of a strong cation exchange (SCX) and a strong anion exchange (SAX) sorbent packed into one bed. AccuCAT is effective for the extraction of acidic, basic and neutral analytes from urine and other biological samples. AccuCAT is particularly effective for catecholamine extraction from bio fluids.

Supplier: VARIAN

Bond Elut™ C2 is a fairly non-polar sorbent because of the short chain length of the functional group. C2 is often used during the process of method development if analytes are retained too strongly on a C8 or C18 phase. The polarity of C2 is slightly lower than a cyano phase for polar interactions.

Supplier: VARIAN

Native silica is generally regarded as the most polar SPE sorbent available. Bond Elut™ SI is particularly effective at separating compounds with a very similar structure. Applying the analytes in a non-polar solvent, then increasing the solvent polarity by increasing the concentration of a polar modifier, such as THF or ethyl acetate, delivers effective separations.

Supplier: VARIAN

Bond Elut™ NH₂ is a weaker anion exchanger than sorbents such as SAX (a quaternary amine sorbent that is always charged) and is therefore a better choice for retention of very strong anions, such as sulfonic acids, which may retain irreversibly on a SAX sorbent. Similar to Diol and SI sorbents, Bond Elut™ NH₂ is excellent for the separation of structural isomers.

Supplier: VARIAN

Bond Elut™ SAX is a strong anion exchange sorbent ideally suited for the extraction of compounds such as carboxylic acids, which may not retain effectively on weak anion exchange sorbents. The sorbent is supplied in the chloride counter ion form. You can modify the selectivity of the phase by replacing the chloride ion with other anions, such as acetate and hydroxide.

Supplier: Cytiva

HiScreen columns prepacked with Blue Sepharose™ Fast Flow has cibacron blue 3G covalently attached to the Sepharose™ 6 Fast Flow matrix by the triazine coupling method.This is an excellent choice for method optimisation and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins.

Supplier: VARIAN

Bond Elut™ DEA bears some resemblance to Bond Elut™ NH₂ in its properties but with a slightly lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C₈ but less polar than C₂ or CN-E.

Supplier: VARIAN

Bond Elut™ PH is a non-polar bonded silica material which exhibits a different selectivity to alkyl or aliphatic functionalised phases such as C8 or cyclohexyl. The electron density present in the aromatic ring affords an enhancement in the retention of conjugated or aromatic ring-containing analytes due to desirable pi-pi interactions.

Supplier: Thermo Fisher Scientific

Protein desalting spin columns are designed to desalt or exchange buffer of protein samples with volumes of 30 to 120 µl. These devices have exceptional desalting characteristics with ≥95% retention of salts and small molecules while providing excellent recovery of proteins greater than 7 kDa. Multiple samples can be processed in less than five minutes without cumbersome column preparation steps.

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Supplier: HICHROM

CIMmultus™ columns are based on an innovative monolithic technology. Distinguished by large pores/flow through channels these columns are designed for the production, purification, and analytics of large proteins (IgG, IgM, PEGylated proteins), viruses (AAV, adenovirus, lentivirus, influenza, bacteriophages), VLPs, exosomes and pDNA for example.

Supplier: Thermo Fisher Scientific

HyperSep™ Retain™-CX polymeric SPE material is a high purity, highly porous, polystyrene DVB material partially functionalised with sulphonic acid groups to give balanced retention of basic and non polar analytes. HyperSep™ Retain™-CX is ideal for the retention of a wide range of drugs of abuse, including basic and neutral drugs.

Supplier: VARIAN

Bond Elut™ C8 is very similar in properties to C18, but is not as retentive for non-polar compounds, due to its shorter hydrocarbon chain, and therefore reduced carbon loading. C8 is an excellent replacement for C18 when analytes are too strongly retained for effective elution. The potential for polar interactions is somewhat higher than for C18 because there is less coverage of the silica surface. These polar interactions are not, however, a significant property of C8.