buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Ambion® Buffer, TE solution (TRIS-EDTA buffer), RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)

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RoboSep™ Buffer 2

Supplier: Stemcell Technologies

RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Ambion

Running Buffer, TAE buffer solution 25X concentrate (TRIS-acetate-EDTA buffer)

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Buffer solutions

Supplier: Hanna

Solutions in sachets ensure optimum precision. The plastic-coated sachet provides a perfect liquid seal and good protection from light. The solutions should only be used once to avoid any danger of contamination. They also satisfy the requirements of the GLP and ISO standards. They have been adjusted using a pH meter that has been calibrated according to the NIST standards.

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Ambion® Buffer, TE solution (TRIS-EDTA buffer) pH 8,0, RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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RoboSep™-C PBS/EDTA Buffer

Supplier: Stemcell Technologies

PBS buffer containing EDTA.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: Ambion

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Fixation Buffer

Supplier: Stemcell Technologies

Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.

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HEPES Buffer Solution (1 M)

Supplier: Stemcell Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

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ALDEFLUOR™ Assay Buffer

Supplier: Stemcell Technologies

Use ALDEFLUOR™ Assay Buffer to optimize the detection of aldehyde dehydrogenase-bright (ALDHbr) cells and maximize fluorescent signal stability during the ALDEFLUOR™ assay.

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Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™

Supplier: Ambion

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)

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EasySep™ Red Blood Cell Lysis Buffer

Supplier: Stemcell Technologies

EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.

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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum

Supplier: Stemcell Technologies

Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.

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Ambion® buffer, sodium acetate buffer solution pH 5,5 (3 mol/L), RNase-free, Invitrogen™

Supplier: Ambion

Cloning Buffer, Sodium acetate buffer solution pH 5,5 (3 mol/l)

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Intracellular Permeabilisation Buffer (10X)

Supplier: Stemcell Technologies

Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.