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buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

 
 
Fixation buffer for microscopy and flow cytometry

Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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Dry buffer packs, Carbonate-Bicarbonate Buffer, JAW™

Supplier: G-Biosciences

Premixed buffer powders that simply require adding deionised water to generate a ready-to-use buffer.

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VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

VWR® Phosphate Buffered Saline (PBS) with 0,05% Tween®20

Supplier: VWR Chemicals

Phosphate Buffered Saline (PBS) with 0,05% Tween® 20, pH 7,5 is a ready-to-use 1× concentration.

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Hybridisation buffers

Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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Lithium nitrate, ionisation buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Lithium Nitrate Buffer Solution

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Caspase cell lysis buffer

Supplier: Enzo Life Sciences

Caspase cell lysis buffer, Enzo Life Sciences

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR®, Rapid Transfer buffer 10X

VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

   Sustainable Options Available
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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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Buffer, PBS (10X, Dulbecco's) powder pack

Buffer, PBS (10X, Dulbecco's) powder pack

Supplier: PanReac AppliChem

Powder mixture to prepare PBS buffer (10X) according to Dulbecco.

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WB blocking buffer kit, TrueBlack®

Supplier: Biotium

The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

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Trizmal™ Buffer, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

For use in the Naphthol AS-D Chloroacetate Esterase Kit No. 91-C.

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Cesium nitrate, ionization buffer solution, Specpure®

Supplier: Thermo Fisher Scientific

Cesium Nitrate Buffer solution

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Buffer, TE solution (TRIS-EDTA buffer) pH 8,0

Supplier: Rockland Immunochemicals

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0

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RIPA cell lysis buffer 2

Supplier: Enzo Life Sciences

RIPA cell lysis buffer 2, Enzo Life Sciences

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