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buffers

Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

 
 

10X Blocking Buffer, Abcam

Supplier: Abcam

10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding. - Cited in over 30 publications - Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB) - 100% casein blocking as preservative

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Blocking buffers

Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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Guanidine hydrochloride buffer solution, 6 mol/L, pH 8,7

Supplier: TCI

Guanidine Hydrochloride Buffer Solution, 6 mol/L, pH value: 8,7, TCI

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Instant Buffers (acc. to Soerensen)

Supplier: TCI

Instant Buffer S64, (acc. to Soerensen), pH value: 6.4

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Cell Lysis Buffer, Abcam

Supplier: Abcam

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

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Native lysis Buffer, Abcam

Supplier: Abcam

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.

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Fixation buffer for microscopy and flow cytometry

Fixation buffer for microscopy and flow cytometry

Supplier: Biotium

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

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Permeabilisation buffer

Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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6X GelRed prestain loading buffer with tracking dye

Supplier: Biotium

6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).

    
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Plasmid DNA buffers

Supplier: OMEGA BIO-TEK

Solution 1, resuspension buffer

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E.Z.N.A.® RNALock stabilizer reagent

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

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Immunoassay Blocking (BSA Free)

Immunoassay Blocking (BSA Free)

Supplier: Abcam

Immunoassay Blocking (BSA Free) (ab171535) maintains the activity of antibodies and other biomolecules adsorbed or immobilized to solid substrates.

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RBC Lysis Buffer (10X)

RBC Lysis Buffer (10X)

Supplier: Tonbo Biosciences

RBC Lysis Buffer (10X) is a concentrated ammonium chloride-based lysing reagent.

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Electrophoresis running buffers

Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.

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G-Biosciences Red Blood Cell Lysis Buffer

Supplier: G-Biosciences

A red blood cell lysis buffer for the isolation of white blood cells free from interference of RBCs. The optimised concentration of ammonium chloride and enhances effectively lyses red blood cells, while preserving the integrity of the leukocytes.

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Immunoassay Blocking Buffer

Immunoassay Blocking Buffer

Supplier: Abcam

Immunoassay blocking buffer effectively preserves the conformation and activity of dried proteins in immunoassays.

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