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1063 results for "SIGMA ALDRICH MICROSCOPY"

1063 Results for: "SIGMA ALDRICH MICROSCOPY"

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Lucifer yellow CH dilithium salt for fluorescence Stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

A highly fluorescent dye, useful in marking nerve cells.

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Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt,powder (Sulfo-SMCC)

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Sulphorhodamine 101 acid chloride, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Sulforhodamine 101 acid chloride has been used in an encapsulated fluorescent probe as a model drug to study drug-delivery properties of rhamnogalacturonan-I based microcapsules. It has also been used in confocal imaging as an astrocyte specific dye.

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Schaeffer and Fulton Spore Stain Solution B, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).
Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water

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PAS (Periodic Acid Schiff) staining kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Standard procedure and microwave procedure included in package insert.
When treated with periodic acid, glycols are oxidized to aldehydes. After reaction with Schiff′s Reagent (a mixture of pararosaniline and sodium metabisulfite), a pararosaniline adduct

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Crystal violet 1% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

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Zinc protoporphyrin guanylate cyclase inhibitor, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Inhibits soluble guanylate cyclase.

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Acid Yellow 25 40% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Yellow 25, Dye content 40 %

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Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

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3,6-Dibutoxy-1,2-benzenedicarbonitrile ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,6-Dibutoxy-1,2-benzenedicarbonitrile,97% (3,6-Dibutoxyphthalonitrile)

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Azure B (azure I) ≥85% (by HPLC), prepared by direct synthesis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule. Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localisation of nucleic acids and lignin.

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Formalin 10% histological fixative, neutral buffered

Supplier: SIGMA ALDRICH MICROSCOPY

10% Neutral buffered formalin (NBF) is the most commonly used fixative for light microscopy. 10% NBF is well suited for large throughput laboratories and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Organo/Limonene Mount™

Organo/Limonene Mount™

Supplier: SIGMA ALDRICH MICROSCOPY

Organo/Limonene Mount™ is a ready-to-use mounting medium is made with limonene, a natural product from orange peels. It is good for coverslipping stained tissues and cell smears that can be dehydrated with organic solvents.

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Immersion oil for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

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Sudan red G in toluene, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Sudan red G in toluene, Sigma-Aldrich®

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trans-4-Hydroxycinnamic acid ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

p-Coumaric acid has been used as a component of chemiluminescent substrate for protein detection in western blotting.

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4-Diazodiphenylamine sulphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Variamine Blue RT Salt has been used as a dye in ultraviolet-visible spectrophotometric method based-uricase enzyme for the detection of uric acid in normal urine. It is also used in combination with naphthol AS-MX phosphate-variam to determine the cytochemical visualization of alkaline phosphatase activity.

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Sigmacote® siliconizing reagent

Supplier: SIGMA ALDRICH MICROSCOPY

Siliconizing reagent for glass and other surfaces. Sigmacote® is a solution of a chlorinated organopolysiloxane in heptane that readily forms a covalent, microscopically thin film on glass.

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Bromocresol green sodium salt 0.04% in aqueous solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol green is a sulfonephthalein dye. It is mainly used for measuring serum albumin. In acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630 nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 3.8) to blue color (5.4). It is widely used as a colloidal pigment.

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Fluorescein 95% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein 95% (dye content), Sigma-Aldrich®

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Diethyl-3,4-pyridinedicarboxylate ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Diethyl 3,4-pyridinedicarboxylate,97% (Cinchomeronic ester)

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Acid Red 183 30% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Red 183, Dye content 30 %

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4-Chloro-7-sulphobenzofurazan ammonium salt, powder

Supplier: SIGMA ALDRICH MICROSCOPY

4-Chloro-7-sulphobenzofurazan ammonium salt, powder

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Chrysoidine G for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chrysoidine G,for microscopy (Bact., Bot., Vit.) (4-Phenylazo-m-phenylenediamine monohydrochloride; Basic Orange 2; Chrysoidine Y).

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Fast Blue RR Salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast blue RR salt has been used in the immunohistochemistry to detect the activity of alkaline phosphatase. It has been used in the procedure of connective tissue staining using picrosirius red.

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Rose Bengal ≥80% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rose bengal,certified by the Biological Stain Commission, Dye content ≥80 % (4,5,6,7-Tetrachloro-2',4',5',7'-tetraiodofluorescein disodium salt; Acid Red 94; Bengal Rose B sodium salt; Rose Bengal sodium salt).

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N,N-Dimethyl-4-nitrosoaniline 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

N,N-Dimethyl-4-nitrosoaniline has been used along with imidazole to detect the formation of singlet oxygen in cells.

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Eriochrome Black T, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eriochrome Black T, Sigma-Aldrich®

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Poly-L-lysine hydrobromide

Supplier: SIGMA ALDRICH MICROSCOPY

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Light green SF yellowish, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish, Sigma-Aldrich®

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