DNase I
Product Details & Documents
Purified DNase I tested to be free of contaminating activity for the degradation of nucleic acids.
- RNase free
- Contains a mixture of four isoenzymes from bovine pancreas (10 units/µl)
DNase I, RNase Free catalyses the degradation of double-stranded DNA into oligonucleotides 1,2 and mononucleotides. This enzyme has been isolated as a mixture of four isoenzymes from bovine pancreas that cut preferentially next to pyrimidine nucleotides.
Reaction Conditions: 40 mM Tris-HCl (pH 7,5), 6 mM MgCl₂, 2 mM CaCl₂, 1 µg DNA and 1 unit enzyme in a 25 µl volume. Incubate 30 minutes at 37 °C.
Caution: For research use only. Not for use in diagnostic procedures.
Specifications
Specifications
- Pk:1
- Pk Info:5000 units
- Cat. No.:AGLS600032
- Form:Premixed
- Formulation:10 mM Tris-HCl (pH 7,5), 10 mM CaCl₂, 10 mM MgCl₂ and 50 % glycerol (v/v). (Dilutions should be made in this buffer.)
- Enzyme name:DNase I
Specifications
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck