Affinity chromatography columns, HisTrap™ FF crude
HisTrap™ FF crude, PP, 90 µm, 7×25 mm, 1 ml, 4 ml/min
Catalog # 29-0486-31
Supplier: Cytiva
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Product Details & Documents
HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
- High binding capacity – binds up to 40 mg his-tag protein/mL resin
- Excellent flow properties – great performance even at high flow rates
- Optimized for unclarified samples – short purification time can minimize potential negative effects, such as degradation and oxidation of proteins
- HiTrap column format – compatible with syringe, pump, or chromatography system such as ÄKTA systems or other FPLC systems
HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. After thorough cell disruption, it is possible to load the unclarified lysate on the column, without precentrifugation and filtration.
Specifications
- Flow rate:4 ml/min
- Particle Size:90 µm
- Capacity:1 ml
- Description:HisTrap™ FF crude
- Seq:02
- Column hardware:PP
- Bed dimensions:7×25 mm
- Cat. No.:29-0486-31
- Max. pressure:5 bar
- Binding capacity:Approx. 40 mg histidine-tagged protein/mL medium
- Storage:4 to 30 °C, 20% Ethanol
- pH range:3 - 12
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