Mouse Sparcl1 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse Sparcl1 in serum, plasma, tissue homogenates, and other biological fluids.

• Ready-to-use ELISA kit
• Detection range: 0,781 to 50 ng/ml
• Sensitivity: 0,469 ng/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Mouse Sparcl1 ELISA kit (A75025) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse Sparcl1 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Sparcl1 antigen. During the incubation, Sparcl1 present in the samples or standards competes with the fixed amount of immobilised Sparcl1 for binding sites on the Biotinylated Anti-Sparcl1 Antibody. The more Sparcl1 present in a sample or standard, the less Biotinylated Anti-Sparcl1 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Sparcl1 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Sparcl1 present in each sample or standard. The concentration of Sparcl1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.