Culture medium kit for establishment and maturation of human pluripotent stem cell-derived choroid plexus organoids.

Take an in vitro approach to human neural biomarker discovery and central nervous system (CNS) permeability with human pluripotent stem cell (hPSC), derived organoids patterned to the choroid plexus, the specialized brain epithelium that forms the blood-cerebrospinal fluid barrier. With a three-dimensional tissue structure and functional barrier epithelium, achieve unprecedented access and control in studying CNS permeability for drug distribution studies, as well as for production and extraction of cerebrospinal fluid (CSF). Using STEMdiff™ Choroid Plexus Differentiation Kit’s simple five-stage protocol, efficiently and reproducibly generate choroid plexus organoids for a variety of downstream applications.

The kit is based on the formulation published by Pellegrini et al., which was awarded a 3Rs Prize for its breakthroughs in animal reduction, replacement, and refinement. Choroid plexus organoid formation is initiated through an intermediate embryoid body (EB) formation step followed by expansion of neuroepithelia and patterning to choroid plexus-like epithelium. After a maturation period, organoids generated using this kit feature cystic structures filled with a fluid resembling CSF and surrounded by an epithelial layer expressing ependymal choroid plexus-specific markers (TTR, CLIC6, AQP1). For extended periods of organoid culture (> 40 days), the components required for organoid maturation can be purchased as STEMdiff™ Choroid Plexus Organoid Maturation Kit.