Serum-free medium for naïve-like human pluripotent stem cells.

Revert primed human embryonic (ES) and induced pluripotent stem (iPS) cells to a naïve-like state and maintain cells in a naïve-like state under hypoxic conditions without bFGF or feeder cells. Developed under license from the Weizmann institute of science, this serum-free cell culture medium produces robust cultures with features of a naïve-like state, such as tightly packed, domed colonies with refractive edges. Human ES/iPS cells cultured in RSeT™ feeder-free ,medium show increased expression of key transcripts associated with the naïve-like state, such as KLF17, KLF2, KLF4, and TFCP2L1, and can either be differentiated or converted back to a primed state by culture in mTeSR™1 and then differentiated. Compatible products that can be used for differentiation include STEMdiff™ definitive endoderm kit, STEMdiff™ SMADi neural induction kit, and STEMdiff™ mesoderm induction medium.