Serum-free, animal component-free semi-solid medium with supplements for selection and cloning of transfected CHO cells.

• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium
• Rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures

ClonaCell™-CHO ACF is a methylcellulose-based semi-solid medium with supplements for the selection and cloning of suspension-adapted CHO cells in serum-free conditions. This medium is animal component-free and contains only recombinant proteins and synthetic components. Single-cell cloning efficiency of CHO cells is higher in this medium than in protein-free media, especially at low plating densities. It does not contain L-glutamine, selection agents, or phenol red and is compatible with dihydrofolate reductase (DHFR) and glutamine synthase (GS) selection systems.