Naphthol Yellow S is a dye that is known for its hazardous nature.

Naphthol Yellow S may be used as an analytical reference standard for the determination of Naphthol Yellow S in: Foodstuffs by solid phase extraction (SPE) and high performance liquid chromatography (HPLC)-diode array detection (DAD)-ion trap time-of-flight tandem mass spectrometry (IT-TOF-MS3) equipped with multiple reaction monitoring (MRM) detection.

Drinks and candies by SPE followed by analysis using HPLC coupled to photodiode array (PDA) detector. Wastewater samples by adsorption column chromatography and embroideries designed by the famous 19th century French painter Emile Bernard.