Specifications

• Conjugation:
  Unconjugated
• Protein function:
  Cytokine
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Mouse
• Storage conditions:
  –20 °C
• Endotoxin content:
  Endotoxin LAL (EU/µg) ≤0,1
• Biological activity:
  Bioactivity assay: Induces secretion of SEAP in HEK-BlueTM IL-12 cells, ED50 ≤15 ng/ml, Bioactivity ≥6,7×10⁴ units/mg
• Gene ID:
  P43432/P43431
• Reconstitution instructions:
  Sterile water at 0.1 mg/ml
• Endotoxin-free:
  N
• Carrier-free:
  Y
• Protease-free:
  N
• Animal-free:
  N
• Protein synonyms:
  NKSF|CLMF
• Protein/peptide name:
  IL-12
• Purity:
  ≥95%
• Molecular weight:
  p35: 21,7 kDa, p40: 35,8 kDa, total: 57,5 kDa
• Sequence:
  p35: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA  
  p40:  MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
• Endotoxin level:
  Low
• Formulation:
  10 mM sodium phosphate, pH 7,5
• Nuclease-free:
  N
• Grade:
  RUO
• Shipping temperature:
  RT
• Tested applications:
  Bioassay