This buffer is 10 mM Tris-HCl and 1 mM disodium EDTA.

• UV absorption - λ: 260 nm; Amax: 0,04 and λ: 280 nm Amax: 0,01
• Contains traces of metal cations

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Tris-EDTA (TE) buffer solution, pH 8,0 may also be used as a washing buffer.