Fluor de Lys®-SIRT2 is a fluorogenic peptide substrate for SIRT2 (human Sirtuin 2).

Based on residues 317-320 of p53 (Gln-Pro-Lys-Lys(Ac)), a site of regul atory acetylation by the PCAF acetyltransferase (lysine 320), it was the best for SIRT2 from among a panel of substrates patterned on p53, Histone H3, and Histone H4 acetylation sites.

Fluor de Lys®-SIRT2 is deacetylated by SIRT2 at a rate of more than 50-fold that of the acetylated lysine substrate, Fluor de Lys® (BML-KI104; acetylated substrates both at 25 µM, 500 µM NAD+).

Must be used in conjunction with Fluor de Lys® Developer II (BML-KI176). Sufficient for 100 assays of human recombinant SIRT2 (BML-SE251; 2U/well, 100 µM substrate).