Pierce™ Ni-NTA chromatography cartridges are convenient, ready to use pre-packed devices for the immobilised metal affinity chromatography purification of polyhistidine-tagged proteins from a soluble protein extract.

• High capacity which can bind up to 60 mg of 6×His-tagged protein per ml of resin
• Purify proteins using native or denaturing conditions
• Same batch of resin can be reused at least five times with no loss in performance

These cartridges contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins. The resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates and performs well in batch-binding and spin-column procedures at a variety of scales.