Specifications

Antibody type:
Primary
Antigen name:
Mouse Double Minute 2
Antigen symbol:
MDM2
Clonality:
Polyclonal
Conjugation:
Unconjugated
Host:
Rabbit
Isotype:
IgG
Reactivity:
Human,
Rat,
Mouse
Western blot:
Yes
Epitope:
C-Terminal
Cross adsorption:
No
Form:
Lyophilized
Antigen synonyms:
1700007J15Rik|Double minute 2 protein|AA415488|HDMX|Ubiquitin-protein ligase E3 Mdm2|Mdm-2|ACTFS|Oncoprotein Mdm2|p53-binding protein Mdm2
Amino acid number:
343 to 358
UniProtKB:
Q00987
Sequence:
EKAKLENSTQAEEGFD
Storage temperature:
–20 °C
Immunogen:
A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2 (343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
Purification:
purified by immunogen affinity purification
Size:
100 µg
Pk:
100 µG

Specifications

About this item

Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human;Mouse;Rat.

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.

Type: Primary
Antigen: MDM2
Clonality:
Clone:
Conjugation:
Epitope: near the C-terminus
Host: Rabbit
Isotype: IgG
Reactivity: Human, Mouse, Rat