About this item
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
- For affinity chromatography
- Recombinant Protein G type 3 coupled to Sepharose CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale
- Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse
- Maleimide activation coupling method
- Yields up to 18 mg/mL mouse IgG
- Stable in all commonly used aqueous buffers
GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.
Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- pH range:3 - 9
- Matrix:Cross-linked agarose, 6%
- Ligand:Recombinant GammaBind G type 3 lacking albumin-binding region
- Storage temperature:2 - 8 °C
- Binding capacity:> 35 mg human IgG/mL
- Flow rate:<130 cm/h