Specifications
- Pk:1 KIT
- Description:InGel™ Array
- Environmentally Preferable:
Specifications
About this item
InGel™ Array is a 96-well format kit suitable for processing larger numbers of protein spots concurrently and is compatible with spot-picking instruments. Fewer than 96 samples a batch may also be processed without any waste.
- Digestion of protein spots in 96 well titre plate format
- Suitable for 2D gels stained with silver stain, Coomassie® dye, and other methods
The protein spots are first excised from the silver stained gel and transferred to a proteomic grade titre plate. Silver stained gel pieces are washed with SilverOUT™ to remove inhibitory silver ions. The proteins are then digested within the gel using a mass spectrometry grade trypsin and a supplied trypsin digestion buffer.
The digested peptides are extracted with G-Biosciences' Pep-Extract™, a high diffusion peptide extraction buffer. The extracted peptides are suitable for mass spectrometry analysis without any subsequent treatments or cleaning procedures.