The Z-Competent™ E. coli transformation kit is designed to generate competent E. coli cells for simple and highly efficient E. coli transformation.

• No heat shock required
• Competent cells in <20 mins
• Transformations in <5 mins
• High efficiency transformations

E. coli are grown in SOB medium (supplied with GZ-5), washed and suspended in supplied competent buffer. The bacterial cells are now ready for transformations. The transformation efficiency is 2×10⁸ to 1×10⁹ transformants per μg of pUC19 plasmid. The efficiency of transformation varies depending on E. coli strain and plasmid size. The competent cells can be used immediately or stored for later use.