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Microscopy and staining reagents

Avantor offers a broad line of reagent droppers, diagnostic kits, and antigens and antisera, for aid in identifying a variety of microscopic organisms. The visualization of bacteria by means of staining procedures is an important tool for any microbiologist to have at their disposal, and these carefully standardized and reproducible stain reagents help to ensure accurate and easily interpretable results. Stains are also available to provide early detection of positive blood cultures by fluorescence procedures.

ImmunoHistoMount™ Mounting Medium

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

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J.T.Baker®, Cervix Fixative

Supplier: Avantor

Microscopy and staining reagents, J.T.Baker®, Cervix spray fixative

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Sigma-Aldrich® Bouin's solution

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

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Non-aqueous DPX mounting medium

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

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Immersion oil for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

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Immersion oil for microscopy, Sigma-Aldrich®

Supplier: Merck

Used to increase lens effectiveness

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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

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Araldite® M, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.

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Mountant for histology, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

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RenLam® M-1 for microscopy, suitable as a embedding medium, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)

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J.T.Baker®, Formalin Neutraliser

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

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Neutral mounting medium, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

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Eukitt® mounting medium

Supplier: SIGMA ALDRICH MICROSCOPY

This is a mounting medium (contains 45% acrylic resin and 55% xylenes) used in microscopy. It is an adhesive and specimen preservative that can be used manually and in automated coverslipping equipment. Special purpose adhesive and sealant for use with glass, non plastic and other non-reactive materials.

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Epoxy Embedding Medium Kit

Supplier: SIGMA ALDRICH MICROSCOPY

The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

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Immersion oil viscosity 1,250 cSt (lit.), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

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HistoChoice® Clearing agent, Sigma-Aldrich®

HistoChoice® Clearing agent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

HistoChoice® is a patented, complex mixture of agents that allow tissues to retain their structure, antigenic sites and nucleic acid sites. This formulation in non-citrus, contains no mercury, formaldehyde or glutaraldehyde.

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VWR® Fixing Solution

VWR® Fixing Solution

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

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Aqueous mounting medium Aquatex®, Sigma-Aldrich®

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

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Aqueous mounting medium, Fluoroshield™ with DAPI, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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Osteo-Bed Bone Embedding Solvent

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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Zinc Formalin Fixative

Supplier: SIGMA ALDRICH MICROSCOPY

Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.

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Immersion oil viscosity 150 cSt (lit.), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

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Mounting Medium, CC/Mount™

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

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M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

Supplier: Merck

M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.

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VWR®, OCT Embedding matrix for frozen sections

VWR®, OCT Embedding matrix for frozen sections

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

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Embedding medium, PolyFreeze, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

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Ren™ HY 956 epoxy embedding medium, hardener, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

to be used with SIAL 78711 RenLam M-1 (Epoxy Embedding Medium)

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Aqueous mounting medium, Fluoroshield™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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VWR® Q Path® Cytofix, Cytology Fixture

VWR® Q Path® Cytofix, Cytology Fixture

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

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