You searched for: Protein Purification

Supplier: G-Biosciences

Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: G-Biosciences

Immobilised Catalase is prepared by covalent coupling of catalase enzyme to 6% cross-linked agarose beads. Immobilised Catalase is used to remove hydrogen peroxide from biological samples for use in downstream applications which require absenceof hydrogen peroxide as it causes interference.

Supplier: Thermo Fisher Scientific

Pierce F(ab')2 preparation kit uses immobilised pepsin protease to selectively digest whole human and other IgG antibodies to make F(ab')2 fragments that retain antigen binding activity.

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

Supplier: Merck Millipore (Novagen)

The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

Supplier: Thermo Fisher Scientific

The methyl-PEG_n-amine (MA[PEG]_n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

Supplier: Thermo Fisher Scientific

Provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

Supplier: Thermo Fisher Scientific

Methyl-PEG₄-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: Thermo Fisher Scientific

Carboxy-PEG₁₂-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Cytiva

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.

Supplier: Cytiva

rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.