Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Immobilisation kit for peptides, SulfoLink™, Pierce™
Supplier: Thermo Fisher Scientific
Immobilisation Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
Expand 1 Items
PEGylated N-succinimidyl S-acetylthioacetate, SAT(PEG)4, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ SAT(PEG)₄ (PEGylated N-succinimidyl S-acetylthioacetate) is a sulphhydryl-addition reagent for covalent modification of primary amines.
Expand 1 Items
Direct magnetic IP/Co-IP kit, Pierce™
Supplier: Thermo Fisher Scientific
The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.
Expand 1 Items
Affinity purification resins, UltraLink™ iodoacetyl resin
Supplier: Thermo Fisher Scientific
UltraLink™ iodoacetyl resin is a durable, porous resin that is activated to enable efficient and direct covalent immoblisation of peptides and other ligands through their sulfhydryl groups (-SH) for use in affinity purification procedures.
Expand 1 Items
Affinity purification kits, Melon™ gel Monoclonal IgG purification kit
Supplier: Thermo Fisher Scientific
Melon™ Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for monoclonal antibodies from ascites or hybridoma cell culture supernatant, or for large scale antibody purification from serum.
Expand 1 Items
AminoLink™ Plus micro immobilisation kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ AminoLink™ Plus Micro immobilisation kit enables covalent attachment of amine-containing ligands for use in antibody purification and other affinity protocols.
Expand 1 Items
Inclusion body solubilisation reagent, Pierce™
Supplier: Thermo Fisher Scientific
Inclusion Body Solubilisation Reagent is designed to retrieve expressed protein in soluble form after lysis and extraction with reagents.
Expand 1 Items
Micro immobilisation kit, GlycoLink™
Supplier: Thermo Fisher Scientific
Hydrazide-activated resin to immobilise glycoproteins for large-scale affinity purification.
Expand 1 Items
NHS-activated agarose, Pierce™
Supplier: Thermo Fisher Scientific
NHS-activated agarose is an amine-reactive, beaded-agarose resin for rapid and stable immobilisation of proteins, peptides and other ligands via primary amines.
Expand 5 Items
Affinity chromatography media, high capacity endotoxin removal resin, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ High Capacity Endotoxin Removal Resins are ideal for the removal of endotoxins from protein or antibody samples prior to cell transfection or animal injection.
Expand 3 Items
Immobilised Streptavidin resin, UltraLink™, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Streptavidin UltraLink™ resin consists of purified recombinant streptavidin that has been covalently immobilised onto a beaded acrylamide support. UltraLink™ beads are hydrophilic, charge free, highly crosslinked, rigid and durable which makes the affinity resin suitable for a variety of batch or column type affinity procedures that require medium pressure chromatography.
Expand 4 Items
Pierce™ Recombinant Protein G, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.
Expand 1 Items
Recombinant protein L, biotinylated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Biotinylated Recombinant Protein L can be used to probe and detect mouse and human antibodies containing kappa light chains in Western blotting, ELISA, IHC and other immunoassay protocols.
Expand 1 Items
Recombinant protein A/G, peroxidase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Peroxidase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
Expand 1 Items
Recombinant protein A/G, alkaline phosphatase conjugated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Recombinant Alkaline Phosphatase Conjugated Protein A/G is useful to probe and detect antibodies, especially IgG isotypes, of many different species hosts in Western blotting, ELISA, IHC and other immunoassay protocols.
Expand 1 Items
GlycoLink™ IP kit
Supplier: Thermo Fisher Scientific
Thermo Scientific™ GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilisation of antibodies and other glycoproteins via oxidised sugar groups.
Expand 1 Items
High Select™ HSA/immunoglobulin depletion resin
Supplier: Thermo Fisher Scientific
Thermo Scientific™ High Select HSA/Immunoglobulin Depletion Resin decreases the albumin and antibody components that are abundant in human plasma samples, helping prepare the samples for mass spectrometry or 1D or 2D electrophoresis.
Expand 1 Items
TCEP solution, neutral pH, Bond-Breaker™ Pierce™
Supplier: Thermo Fisher Scientific
Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
Expand 1 Items
Pegylation reagents, Methyl-PEG-NHS-Esters, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ MS(PEG)n reagents are methyl-terminated, polyethylene glycol compounds (n equals 4 to 24 PEG units) and TMS(PEG)12 is a three-branched, polyethylene glycol compound (3× 12 PEG units). Both are activated as NHS esters for covalent pegylation of primary amines on proteins (eg. lysines) or assay surfaces.
Expand 7 Items
Glutathione magnetic agarose, Pierce™
Supplier: Thermo Fisher Scientific
These beads provide a fast, convenient method for purification of glutathione-S-transferase (GST) from a bacterial, yeast, or mammalian crude cell lysate.
Expand 2 Items
Protein purification system, ÄKTA™ start
Supplier: Cytiva
ÄKTA start is an easy to use chromatography system that automates manual purification procedures using, for example, HiTrap columns. It offers an entry level chromatography solution for the preparative purification of proteins at lab scale.
Expand 1 Items
Agarose coupling kits, HOOK™
Supplier: G-Biosciences
The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.
Expand 2 Items
Coupling catalyst, GlycoLink™
Supplier: Thermo Fisher Scientific
An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
Expand 1 Items
Control agarose resin, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Control Agarose Resin is underivatised beaded agarose support for pre-clearing protein immunoprecipitation samples.
Expand 1 Items
Direct IP kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Direct IP Kit uses an activated resin to covalently immobilise IP antibodies (any species or class) on agarose beads without the aid of Protein A/G, enabling immunoprecipitation without antibody interference.
Expand 1 Items
Recombinant streptavidin
Supplier: G-Biosciences
Recombinant streptavidin from Streptomyces avidinii, produced in E.coli.
Expand 3 Items
Recombinant protein G, peroxidase conjugated, Calbiochem®
Supplier: Merck Millipore (Oncogene)
Purified recombinant Protein G conjugated to peroxidase can be used in direct immunoassay.
Expand 1 Items
HCCA matrix
Supplier: BRUKER DALTRONIK
Pre-portioned HCCA matrix provides easy and convenient preparation of HCCA matrix solutions.
Expand 1 Items
BTSNM support materials, Chelex beads, 0,4 g with buffer
Supplier: G-Biosciences
Chelex beads are made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.
Expand 1 Items
Streptavidin agarose
Supplier: Merck Millipore (Novagen)
Streptavidin agarose consists of cross-linked agarose covalently coupled with pure streptavidin under controlled conditions. The stable linkage to the resin minimizes leaching of the streptavidin while maintaining full binding activity. The matrix is suitable for use in column and batch formats for any application that requires high biotin binding capacity and low non-specific binding. The resin has no detectable protease, DNase, or RNase contamination.