You searched for: Protein Purification

Supplier: Thermo Fisher Scientific

Pierce™ Protein A/G UltraLink™ and Protein A/G Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which recombinant Protein A/G has been immobilised for use in IgG purification methods. Immoblised proteins are covalently coupled to an agarose support to provide an efficient method of purifying antibodies.

Supplier: Thermo Fisher Scientific

SulfoLink™ Immobilisation Kit for Proteins is a kit containing all the reagents for preparing the protein sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Cobalt Superflow Agarose is a tetradentate-chelating Superflow 6 support charged with divalent cobalt, designed for high-purity, large-scale purification of polyhistidine-tagged proteins.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

Pierce™ Anti-c-Myc Agarose is a high-affinity immunoprecipitation resin ideal for purification of recombinant c-Myc-tagged proteins expressed in human in vitro expression systems and bacterial and mammalian cell lysates.

Supplier: Thermo Fisher Scientific

NeutrAvidin™ agarose is a beaded agarose resin of immobilised NeutrAvidin™ protein, a modified form of avidin with exceptional biotin-binding characteristics for affinity purification methods.

Supplier: Thermo Fisher Scientific

Pierce™ Iminobiotin Agarose is for the purification of avidin, streptavidin or Thermo Scientific NeutrAvidin® Conjugates. Iminobiotin is a cyclic guanido analogue of biotin and has a lower affinity constant for binding avidin, streptavidin or NeutrAvidin® Protein.

Supplier: Thermo Fisher Scientific

HisPur™ Ni-NTA resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

The Pierce™ IgM Purification Kit is an easy to use affinity matrix and buffer system to purify IgM from mouse ascites fluid. The method uses immobilised mannan binding protein (MBP), which binds to IgM in the kit-supplied buffers.

Supplier: Thermo Fisher Scientific

Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules. Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions.

Supplier: Thermo Fisher Scientific

AminoLink™ Plus immobilisation kit contains AminoLink™ Plus coupling resin and additional reagents to perform five protein immobilisations, with expected yields of 2 to 40 mg protein per column. The coupling resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

Supplier: Thermo Fisher Scientific

Protein A UltraLink™ and Protein A Plus UltraLink™ resins are extremely durable beaded polyacrylamide supports to which IgG-binding Protein A has been immobilised for use in IgG purification methods. Protein A resin is used to bind and purify IgG in gravity-flow, spin-column or medium pressure chromatography methods.

Supplier: Thermo Fisher Scientific

Pierce™ HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

Supplier: Thermo Fisher Scientific

Pierce™ chicken IgY purification kit purifies chicken antibodies from hen egg yolks using a unique delipidation reagent to separate protein from lipid and a precipitation reagent to selectively isolate the IgY fraction.

Supplier: Thermo Fisher Scientific

Pierce™ Protein G UltraLink™ and Protein G Plus UltraLink™ resins are useful for purification of antibodies and immunoprecipitation (IP). Samples containing IgG are incubated with the resin-immobilised Protein G in a buffer that facilitates binding. After non-IgG and non-antigen components of the sample are washed from the resin, the bound IgG and antigen may be recovered by elution.

Supplier: Thermo Fisher Scientific

The Pierce™ NAb™ Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilised protein resin enables quick purification of 100 to 1000 µg of IgG from 25 to 500 µl of serum or other sample. The actual amount of IgG purified varies depending upon the sample type and the specific spin column used.

Supplier: Thermo Fisher Scientific

AminoLink™ coupling resin is crosslinked, 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilisation of antibodies and other proteins through primary amines.

Supplier: Thermo Fisher Scientific

The Pierce™ Protein Refolding Kit includes high-purity reagents and detailed instructions for using a matrix strategy to determine optimal buffer conditions for refolding recombinant proteins that have been denatured and solubilised from inclusion bodies.

Supplier: Thermo Fisher Scientific

Pierce™ High capacity streptavidin agarose consists of purified recombinant streptavidin that has been covalently immobilised at a high density onto high-quality crosslinked 6% beaded agarose.

Supplier: Thermo Fisher Scientific

AminoLink™ Immobilisation Kit contains AminoLink™ coupling resin and associated reagents sufficient to perform 5 protein immobilisations, with an expected yield of 2 to 20 mg protein per column.

Supplier: Thermo Fisher Scientific

Pierce SDS-PAGE sample prep kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Supplier: Thermo Fisher Scientific

Pierce™ F(ab')2 micro preparation kit enables efficient Fab generation from IgG.

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein L can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Supplier: Thermo Fisher Scientific

Pierce™ Recombinant Peroxidase Conjugated Protein A can be used to probe and detect rabbit and human antibodies, especially IgG isotypes, in Western blotting, ELISA, IHC and other immunoassay protocols.

Supplier: Thermo Fisher Scientific

Pierce™ Fab preparation kits use immobilised papain protease to digest human or mouse IgG antibodies to make separate Fab and Fc fragments and subsequently to purify the Fab using Protein A agarose.

Supplier: Thermo Fisher Scientific

Pierce™ MM(PEG)_n reagents are methyl-terminated PEG compounds activated with a maleimide group for covalent pegylation of sulfhydryls on proteins or assay surfaces.

Supplier: 3M

3M™ Polisher ST is a fully encapsulated anion exchange (AEX) product to reduce host sell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography of biopharmaceutical process streams.

Supplier: Thermo Fisher Scientific

The Pierce™ Mouse IgG1 Fab and F(ab')2 Preparation Kits use immobilised ficin protease to efficiently digest mouse IgG1 into Fab or F(ab')2 fragments.

Supplier: Thermo Fisher Scientific

Pierce™ HA-Tag IP/Co-IP kit provides the affinity resin, positive control and other reagents necessary to perform immunoprecipitation (IP) or co-immunoprecipitation (Co-IP) reactions with an HA-tagged bait protein.