You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT Bioquest
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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MMP Red™ Substrate
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
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Amplite® Colorimetric Glucose Oxidase Assay Kit
Supplier: AAT Bioquest
The glucose oxidase is a dimeric protein that catalyses the oxidation of beta-D-glucose into hydrogen peroxide and D-glucono-1,5-lactone, which is hydrolysed to gluconic acid.
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Amplite® Fluorimetric Beta-Hydroxybutyrate (Ketone Body) Assay Kit
Supplier: AAT Bioquest
Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop.
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Amplite® Colorimetric Gama-Glutamyltransferase (GGT) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Gamma-Glutamyltransferase (GGT) Activity Assay Kit provides an efficient and straightforward method for measuring GGT activity across various sample types.
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Pierce™ Cypridina Luciferase Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.
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Amplite® Colorimetric Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® colorimetric GAPDH activity assay kit is a sensitive and simple tool for monitoring GAPDH activity based on a coupled enzymatic reaction catalysed by GAPDH.
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Amplite® Rapid Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*
Supplier: AAT Bioquest
Peroxynitrite (ONOO-) is a strong oxidizing species and a highly active nitrating agent.
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Amplite® Fluorimetric Lead (II) Ion Quantitation Kit
Supplier: AAT Bioquest
Lead is a highly poisonous metal to human beings, especially young children.
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Gaussia luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Gaussia Luciferase Glow Assay kit provides an extremely bright and stable bioluminescence signal (half-life greater than one hour), especially in the presence of Gaussia-Dura luciferase reporter.
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Amplite® Fluorimetric Endotoxin Detection Kit
Supplier: AAT Bioquest
Lipopolysaccharide (LPS), also known as endotoxin, is the major component of the outer membrane of Gram-negative bacteria.
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Amplite® Rapid Colorimetric Maleimide Quantitation Kit
Supplier: AAT Bioquest
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules.
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Amplite® Fluorimetric DPP4 Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Fluorimetric DPP4 Inhibitor Screening Kit provides an efficient and direct method for evaluating potential DPP4 inhibitors.
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Pierce™ Alkaline Phosphatase, Biotinylated
Supplier: Thermo Fisher Scientific
Biotinylated alkaline phosphatase is most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method.
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Pierce™ Firefly Luc One-Step Glow Assay Kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Firefly One-Step Luc Glow Assay Kit measures firefly luciferase activity in mammalian cell culture using a single reagent-addition step, making it ideal for high-throughput screening (HTS) applications.
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Amplite® Human Serum Albumin (HSA) Site I Binding Assay Kit
Supplier: AAT Bioquest
Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.
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Amplite® Fluorimetric Acetylcholine Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Acetylcholine and its metabolites are needed for three main physiological purposes: structural integrity and signaling roles for cell membranes, cholinergic neurotransmission (acetylcholine synthesis), and as a major source for methyl groups via its metabolite, trimethylglycine (betaine) that participates in the S-adenosylmethionine synthesis pathways.
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Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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SAM Methyltransferase assay, SAM510™
Supplier: G-Biosciences
SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Fluorescent β-Galactosidase assay kit
Supplier: G-Biosciences
The Fluorescent β-Galactosidase assay is used for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The Fluorescent β-Galactosidase assay (MUG) provides a 96-well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460 nm.
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Kinase enrichment kits, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.
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Protease Screening Kit
Supplier: G-Biosciences
The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.
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Active GTPase pull-down and detection kits
Supplier: Thermo Fisher Scientific
The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.
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Fluorescent β-Galactosidase assay kit
Supplier: G-Biosciences
β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.
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LOX assay kit (fluorometric)
Supplier: Abnova
LOX assay kit (fluorometric) is designed for the measurement of LOX.
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Alkaline phosphatase assay kit
Supplier: Abnova
Alkaline phosphatase assay kit allows the colorimetric kinetic determination of serum alkaline phosphatase activity.
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Superoxide dismutase assay kit
Supplier: Abnova
Superoxide Dismutase (SOD) Assay Kit is used for the determination of SOD in blood, cells, tissues and other biological samples.
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Second messenger quantitation assays, Biotrak™ cAMP and cGMP EIA systems
Supplier: Cytiva
Eliminate lengthy sample extraction procedures using direct assay technology.
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