You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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DPPIV Drug discovery kit
Supplier: Enzo Life Sciences
The DPPIV Drug Discovery Kit is a complete assay system designed to screen DPPIV inhibitors, a potential therapeutic target, providing enough material to perform at least 96 assays.
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Fluorometric drug discovery assay kit, SIRT5, Fluor-de-Lys®
Supplier: Enzo Life Sciences
The SIRT5 Fluorometric Drug Discovery Kit is a sensitive fluorometric assay is designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5.
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Granzyme B assay kit for drug discovery
Supplier: Enzo Life Sciences
The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.
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Matrix Metalloproteinase-2 (MMP-2) fluorometric drug discovery kit
Supplier: Enzo Life Sciences
A Quantizyme™ Assay System. The MMP-2 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen inhibitors of matrix metalloproteinase-2 (MMP-2, gelatinase A) using a quenched fluorogenic peptide: OmniMMP™ fluorogenic substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-2, a potential therapeutic target.
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SAM Methyltransferase assay, SAMfluoro™
Supplier: G-Biosciences
The SAMfluoro™ assay is a fluorescent, continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.
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Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Colorimetric Phosphofructokinase (PFK) Activity Assay Kit provides a simple and direct procedure for precise measurement of PFK enzyme activity in various biological samples.
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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.
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Amplite® Colorimetric Glycerol Assay Kit
Supplier: AAT Bioquest
Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.
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Amplite® Colorimetric BCA Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional BCA protein assay is widely used for quantifying proteins.
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Amplite® Colorimetric Glucose-6-Phosphate Assay Kit
Supplier: AAT Bioquest
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric L-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.
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Amplite® Colorimetric Glucose Quantitation Kit
Supplier: AAT Bioquest
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT Bioquest
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.
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Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT Bioquest
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Fluorimetric Alanine Aminotransferase Assay Kit
Supplier: AAT Bioquest
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*
Supplier: AAT Bioquest
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*
Supplier: AAT Bioquest
Formaldehyde is a naturally occurring substance.
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Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Amplite® Colorimetric Total NAD and NADH Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric NAD/NADH Ratio Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Yeast β-galactosidase assay kit, Pierce™
Supplier: Thermo Fisher Scientific
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.
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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Supplier: AAT Bioquest
Site-selective modification of proteins is of major interest in chemical biology.
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Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Supplier: AAT Bioquest
Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.