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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

CytoScan™ Cell proliferation assay kit, WST-1

CytoScan™ Cell proliferation assay kit, WST-1

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

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CytoTell™ Blue

CytoTell™ Blue

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyse heterogeneous cell populations.

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CYTO-13

CYTO-13

Supplier: AAT Bioquest

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analysing DNA content in living cells.

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MitoLite™ Blue FX490

MitoLite™ Blue FX490

Supplier: AAT Bioquest

MitoLite™ blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient.

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MitoLite™ Red FX600

MitoLite™ Red FX600

Supplier: AAT Bioquest

MitoLite™ Red is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient.

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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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MitoLite™ Orange FX570

MitoLite™ Orange FX570

Supplier: AAT Bioquest

MitoLite™ orange is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Supplier: MOLECULAR DEVICES

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Cardiotoxicity kit, EarlyTox™

Cardiotoxicity kit, EarlyTox™

Supplier: MOLECULAR DEVICES

The EarlyTox™ Cardiotoxicity Kit provides a fast, simple and reliable fluorescence based method for identifying cardiotoxic compounds in a biorelevant assay using stem-cell derived cardiomyocytes.

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Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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Annexin V, TRITC Labeled

Annexin V, TRITC Labeled

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Annexin V-Cy7 Conjugate

Annexin V-Cy7 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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MitoLite™ Green FM

MitoLite™ Green FM

Supplier: AAT Bioquest

MitoLite™ Green FM is the same molecule to the MitoTracker Green FM (M7514, ThermoFisher).

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CytoTell™ Red 650

CytoTell™ Red 650

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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Annexin V, XFD350 Conjugate

Annexin V, XFD350 Conjugate

Supplier: AAT Bioquest

XFD350 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 350 (Alexa Fluor® is the trademark of ThermoFisher).

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CytoScan™-fluoro cytotoxicity assay kit

CytoScan™-fluoro cytotoxicity assay kit

Supplier: G-Biosciences

The CytoScan™-fluoro Cytotoxicity assay is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluorescent compound (resazurin) to a fluorescent compound (resorufin), which is measured using a fluorometer. The assay detects even low level damage to cell membrane not detected with other methods.

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LysoBrite™ NIR

LysoBrite™ NIR

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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LysoBrite™ Orange

LysoBrite™ Orange

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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Annexin V, Recombinant

Annexin V, Recombinant

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Cell viability assay kits, OZBlue

Cell viability assay kits, OZBlue

Supplier: OZ BIOSCIENCES

OZBlue cell viability kit is a ready to use assay system based on the fluorimetric/colorimetric quantification of metabolic activity in living cells. Resazurin dye is used as an indicator of cell viability.

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Oxysterol derivatization kit MaxSpec®

Oxysterol derivatization kit MaxSpec®

Supplier: Cayman Chemical

Derivatization Kit for the sensitive LC-MS analysis of oxysterols.

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LysoBrite™ Red

LysoBrite™ Red

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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CYTO-13

CYTO-13

Supplier: AAT Bioquest

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells.

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LRB Red™ Succinimidyl ester

LRB Red™ Succinimidyl ester

Supplier: AAT Bioquest

Although Lissamine Rhodamine B chloride is one of the most popular labeling reagents of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Lissamine Rhodamine B chloride tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. LRB Red™ is a succinimidyl ester and has the spectral properties identical to those of Lissamine Rhodamine B chloride. LRB Red™ is a superior replacement for Lissamine Rhodamine B chloride. LRB Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Lissamine Rhodamine B chloride, LRB Red™ has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using LRB Red™ to replace Lissamine Rhodamine B chloride for labeling peptides and oligonucleotides.

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Annexin V, PacBlue Conjugate

Annexin V, PacBlue Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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CytoTell™ Red 590

CytoTell™ Red 590

Supplier: AAT Bioquest

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations.

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Nuclear Green™ LCS1

Nuclear Green™ LCS1

Supplier: AAT Bioquest

Our Nuclear Green™ LCS1 is a fluorogenic, DNA-selective and cell-permeant dye for analysing DNA content in living cells.

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LysoBrite™ Blue

LysoBrite™ Blue

Supplier: AAT Bioquest

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

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Forte Blue™ DHPE

Forte Blue™ DHPE

Supplier: AAT Bioquest

Forte Blue™ DHPE is the same molecule to Marina Blue® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (Marina Blue® DHPE, Marina Blue® is the trademark of thermoFisher).

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MitoLite™ Green EX488

MitoLite™ Green EX488

Supplier: AAT Bioquest

MitoLite™ Green is a cationic dye that selectively accumulates in mitochondria probably vial the mitochondrial membrane potential gradient.

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