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Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Screen Quest™ Luminometric Calcium Assay Kit

Screen Quest™ Luminometric Calcium Assay Kit

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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MycoLight™ Red JJ94 *2,5 mM in DMSO*

MycoLight™ Red JJ94 *2,5 mM in DMSO*

Supplier: AAT Bioquest

Green fluorescent protein (GFP) derivatives are widely used as fluorescent reporters to study biological processes at the cellular and molecular level, including labeling bacteria.

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Annexin V-mFluor™ Violet 540 Conjugate

Annexin V-mFluor™ Violet 540 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Annexin V-iFluor® 488 Conjugate

Annexin V-iFluor® 488 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Annexin V-iFluor® 594 Conjugate

Annexin V-iFluor® 594 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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PhosphoWorks™ Luminometric ATP Assay Kit, Steady Glow

PhosphoWorks™ Luminometric ATP Assay Kit, Steady Glow

Supplier: AAT Bioquest

Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling.

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Cell Meter™ Live Cell ATP Assay Kit

Cell Meter™ Live Cell ATP Assay Kit

Supplier: AAT Bioquest

Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling.

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PhosphoWorks™ Luminometric ATP Assay Kit, Bright Glow

PhosphoWorks™ Luminometric ATP Assay Kit, Bright Glow

Supplier: AAT Bioquest

Adenosine triphosphate (ATP) plays a fundamental role in cellular energenics, metabolic regulation and cellular signaling.

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Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

Cell Meter™ Fluorimetric Cell Cytotoxicity Assay Kit

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Screen Quest™ Rhod-4 No Wash Calcium Assay Kit

Screen Quest™ Rhod-4 No Wash Calcium Assay Kit

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence*

PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

ADP is involved in many biological reactions such as protein kinases.

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Cell Meter™ 2-NBDG Glucose Uptake Assay Kit

Cell Meter™ 2-NBDG Glucose Uptake Assay Kit

Supplier: AAT Bioquest

Glucose metabolism, a process which converts glucose into energy, is a primary source of energy supply in most organisms.

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Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*

Cell Meter™ Autophagy Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins, organelles, and other cytoplasmic components for degradation via the lysosomal pathway.

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PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Blue Fluorescence

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit, Blue Fluorescence

Supplier: AAT Bioquest

Pyrophosphate (PPi) is produced by a number of biochemical reactions, such as ATP hydrolysis, DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase and the enzymatic activation of fatty acids to form their coenzyme A esters.

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PhosphoWorks™ Fluorimetric Phosphate Assay Kit, Red Fluorescence

PhosphoWorks™ Fluorimetric Phosphate Assay Kit, Red Fluorescence

Supplier: AAT Bioquest

Cells utilise a wide variety of phosphate (Pi) and polyphosphate esters as enzyme substrates, second messengers, membrane structural components and vital energy reservoirs.

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Screen Quest™ No Wash Potassium Channel Assay Kit

Screen Quest™ No Wash Potassium Channel Assay Kit

Supplier: AAT Bioquest

Potassium (K⁺) ion channel plays an important role in regulating fundamental biological processes including heart rate, hormone and neurotransmitter secretion, water and electrolyte balance.

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Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit *Triple Fluorescence Colors*

Cell Meter™ Live Cell Caspase 3/7 and Phosphatidylserine Detection Kit *Triple Fluorescence Colors*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.

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Annexin V-iFluor® 680 Conjugate

Annexin V-iFluor® 680 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Annexin V-iFluor® 350 Conjugate

Annexin V-iFluor® 350 Conjugate

Supplier: AAT Bioquest

Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium.

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Cell Meter™ RX700 Fixable Viability Dye

Cell Meter™ RX700 Fixable Viability Dye

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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Cell Meter™ Fixable Viability Dye, BX650

Cell Meter™ Fixable Viability Dye, BX650

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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Cell Meter™ BX590 Fixable Viability Dye

Cell Meter™ BX590 Fixable Viability Dye

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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Cell Meter™ VX450 Fixable Viability Dye

Cell Meter™ VX450 Fixable Viability Dye

Supplier: AAT Bioquest

Discrimination and exclusion of dead cells from live cells allows cleaner separation and identification of cell populations.

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Spexyte™ Micro pH Probe *Optimized for Nanodrop*

Spexyte™ Micro pH Probe *Optimized for Nanodrop*

Supplier: AAT Bioquest

Biological samples are usually in a very narrow pH range from 4.5 to 8.5, and many of them need to be provided in micro-volume (microliter).

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CytoFix™ Red Mitochondrial Stain

CytoFix™ Red Mitochondrial Stain

Supplier: AAT Bioquest

CytoFix™ Red mitochondrial stain is a dye that selectively stains mitochondria independent of mitochondrial membrane potential gradient.

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Screen Quest™ Membrane Potential Assay Kit, Orange Fluorescence

Screen Quest™ Membrane Potential Assay Kit, Orange Fluorescence

Supplier: AAT Bioquest

Membrane potential is the difference in voltage between the interior and exterior of a cell.

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CytoScan™ LDH Cytotoxicity Assay kit

CytoScan™ LDH Cytotoxicity Assay kit

Supplier: G-Biosciences

CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.

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Helix Fluor™ 575 Succinimidyl Ester

Helix Fluor™ 575 Succinimidyl Ester

Supplier: AAT Bioquest

FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs.

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ReadiView™ Green/Red Viability Stain

ReadiView™ Green/Red Viability Stain

Supplier: AAT Bioquest

ReadiView™ Green/Red Viability stain is a robust cell stain optimised for staining nucleated cells in Countess II FL or Countess 3 FL instruments.

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CytoWatch™ QZ100 Monocyte Blocking Reagent

CytoWatch™ QZ100 Monocyte Blocking Reagent

Supplier: AAT Bioquest

Some dye-labeled fluorescent antibody conjugates used in cell surface staining of live cells often exhibit non-specific binding in monocytes and macrophages.

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