You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
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ZnAF-2.tetrahydrochloride
Supplier: Enzo Life Sciences
Fluorescent probe for Zn2+
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SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
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CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
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Gold 525 dUTP (lyophilised)
Supplier: Enzo Life Sciences
Gold 525 dUTP (lyophilised)
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CF® Dye cAMP
Supplier: Biotium
Fluorescently labelled cAMP analogs that can be used to probe cAMP receptors.
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R-Phycoerythrin (RPE)
Supplier: Columbia Biosciences
Many applications and instruments were developed specifically for R-phycoerythrin.
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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin
Supplier: Biotium
Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.
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Organelle-ID™ RGB reagent II
Supplier: Enzo Life Sciences
Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids
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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates
Supplier: Biotium
CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.
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Tyramide amplification buffer plus
Supplier: Biotium
An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.
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Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.
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Green 496 dUTP (lyophilized)
Supplier: Enzo Life Sciences
Green 496 dUTP (lyophilised)
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Aqua 431 dUTP (lyophilised)
Supplier: Enzo Life Sciences
Aqua 431 dUTP (lyophilised)
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HRP-conjugate stabiliser, SeramunStab® STA2
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.
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ZnAF-2 DA
Supplier: Enzo Life Sciences
Fluorescent probe for Zn²⁺. Cell permeable.
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ExoBrite™ True EV Membrane Stains
Supplier: Biotium
Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.
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Reactive quencher dyes
Supplier: Biotium
Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.
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Organelle-ID™ RGB reagent I
Supplier: Enzo Life Sciences
Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling
Supplier: Biotium
The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.
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B-Phycoerythrin (BPE)
Supplier: Columbia Biosciences
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
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Dye conjugates dUTP CF®
Supplier: Biotium
CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Biotin SE protein labelling kit
Supplier: Biotium
This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.
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Fluorescein-12-ddUTP
Supplier: Enzo Life Sciences
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
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HRP-conjugate stabiliser, SeramunStab® STNP
Supplier: Apollo Scientific
Liquid product red odourless pH 6,7 to 6,9. Protein free Stabiliser. Enzyme activity preservation by special chemistry.
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Biotin kits, Micro HOOK™
Supplier: G-Biosciences
The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.
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Bio-16-ddUTP, tetralithium salt
Supplier: Enzo Life Sciences
Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.