You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Proteometer-L Fluorescent Reagent
Supplier: NOVILYTIC
Set of 7 reagent vials for use with the Proteometer-L kit.
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Proteometer-CV Full Kit Reagent (300)
Supplier: NOVILYTIC
Fluorescent reagent portion of the Proteometer-CV full kit. This is sold individually due to shipping and storage conditions.
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DIGE buffer kit
Supplier: Cytiva
DIGE buffer kit is a tool to increase the quality, reproducibility, and convenience of 2-D DIGE using the Ettan™ DIGE system. The DIGE buffer kit is a specially designed two-buffer system for running up to twelve DIGE gels simultaneously.
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Proteometer-L Starter Kit Reagent (200)
Supplier: NOVILYTIC
Fluorescent reagent for the Proteometer-L Starter Kit. This is sold separately due to shipping conditions.
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pH buffer solutions, referenced at 20 °C, traceable to SRM from NIST, Fluka™
Supplier: Honeywell Chemicals
pH Buffer solution, Citrate, pH value: 2,00, ±0.02, Reference temperature: 20 °C, Fluka™, 6×1 L
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TISAB solution for Fluoride Determination, Supelco®
Supplier: Merck
TISAB solution for fluoride determination
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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TISAB-III solution for fluoride determination, Supelco®
Supplier: Merck
TISAB III solution for fluoride determinations
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His buffer kit
Supplier: Cytiva
His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.
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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3
Supplier: Thermo Fisher Scientific
Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)
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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®
Supplier: Merck Millipore (Oncogene)
Developed for use in immunoassay applications.
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PBS, 10X solution, molecular biology grade, ultrapure
Supplier: Thermo Fisher Scientific
Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure
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Low pH SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.
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TISAB I Solution, Supelco®
Supplier: Merck
TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.
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Buffer solution for chlorine determination
Supplier: Hach
For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.
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EDTA solution, 0,5 M, molecular biology grade, Calbiochem®, Millipore®
Supplier: Merck Millipore (Calbiochem)
Sterile-filtered solution of 0,5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC). Used in molecular biology applications.
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2-D Protein Extraction Buffer
Supplier: Cytiva
Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
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Blocking reagents, Blotto
Supplier: Rockland Immunochemicals
Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.
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Proteometer-CV Reconstitution Reagent
Supplier: NOVILYTIC
Proteometer-CV reconstitution reagent.
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SSC 20X buffer, MP Biomedicals
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:
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TISAB II Solution, Supelco®
Supplier: Merck
TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.
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CSPL buffer
Supplier: OMEGA BIO-TEK
Component of the Mag-Bind® plant DNA 96 plus kit.
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Ab buffer kit
Supplier: Cytiva
Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.
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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure
Supplier: Thermo Fisher Scientific
TBS is used in Western blotting protocols.
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Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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Y-PER™ plus Yeast Protein Extraction Reagent, Dialysable
Supplier: Thermo Fisher Scientific
This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.
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Protein A/G HP SpinTrap™ Buffer Kit
Supplier: Cytiva
Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.
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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals
Supplier: MP Biomedicals
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.