Tampones
Al resistir los cambios de pH, los tampones protegen las muestras al añadir ácidos o álcalis. Los tampones en polvo y líquidos rastrean eficazmente las proteínas durante el aislamiento y la separación por electroforesis. Los tampones salinos también se pueden utilizar para calibrar y estandarizar medidores de pH y obtener lecturas precisas. Las sustancias bloqueantes impiden la adhesión de los anticuerpos a los sitios libres de la membrana, lo que produciría un fondo interferente. En diversas concentraciones para adaptarse a cualquier necesidad, los tampones pueden presentarse en formato líquido o deshidratado.
TBE tampón 10x (TRIS-borato-EDTA tampón), 10x solución acuosa concentrada
Supplier: Ambion
Running Buffer, TBE tampón 10x (TRIS-borato-EDTA tampón), 10x solución acuosa concentrada
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RoboSep™ Buffer 2
Supplier: Stemcell Technologies
RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.
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TAE tampón 10x (TRIS-acetato-EDTA tampón), 10x solución acuosa concentrada
Supplier: Ambion
Running Buffer, TAE tampón 10x (TRIS-acetato-EDTA tampón), 10x solución acuosa concentrada
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TAE tampón 25x (Tris-acetato-EDTA tampón), 25x solución acuosa concentrada
Supplier: Ambion
Running Buffer, TAE tampón 25x (Tris-acetato-EDTA tampón), 25x solución acuosa concentrada
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Ion pair reagents for analytical HPLC, PIC®
Supplier: WATERS
Tetrabutylammonium hydrogen sulfate, low UV reagent
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Dulbecco's Phosphate Buffered Saline (D-PBS), 10X Concentrate without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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Ambion® buffer, potassium acetate, 3M™ solution, pH 5,5, RNase-free, Invitrogen™
Supplier: Ambion
Cloning Buffer, Potassium acetate buffer solution pH 5,5 (3 mol/l)
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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum
Supplier: Stemcell Technologies
Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Ambion® Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l), Invitrogen™
Supplier: Ambion
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
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SSC solución tampón 20x concentrado (socio citrato salino)
Supplier: Ambion
Hybridisation Buffer, SSC solución tampón 20x concentrado (socio citrato salino)
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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Ammonium Chloride Solution
Supplier: Stemcell Technologies
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.
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SDS Protein Gel Loading Solution, 2X
Supplier: MARKET SOURCE PART PROCESS
SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualise progress of the electrophoresis run by monitoring the location of the blue dye front.
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