Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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TRIS-buffered saline (TBS, 20X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 20X)
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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™
Supplier: HyClone products (Cytiva)
HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.
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Alkaline running buffer solution
Supplier: Thermo Fisher Scientific
Running Buffer, Alkaline running buffer solution
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Phosphate, 0,5M buffer solution, pH 7,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,5 M, pH value: 7,5, Liquid
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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MOPS-EDTA-sodium acetate buffer (MESA)
Supplier: Thermo Fisher Scientific
MOPS-EDTA-sodium acetate buffer (MESA), Liquid
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TRIS-buffered saline (TBS, 10X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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Hybridisation buffers, formamide-free, HYB-LINK™
Supplier: G-Biosciences
Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.
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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free
Supplier: Thermo Fisher Scientific
Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid
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MES-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MES-SDS running buffer (20X)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®
Supplier: Corning
20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures
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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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Separating or resolving buffer (4X)
Supplier: Thermo Fisher Scientific
Separating Buffer, Separating or Resolving Buffer, 4X
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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