Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Nitrocellulose stripping buffer (10X)

Supplier: Thermo Fisher Scientific

Electrophoresis, Nitrocellulose Stripping Buffer, 10X

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Buffer, ACES, 0,5 M solution

Supplier: Thermo Fisher Scientific

ACES, 0,5M buffer solution, pH 6,5, Colourless, Liquid

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MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

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TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

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TRIS-buffered saline (TBS, 10X) pH 7,6

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

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Phosphate, 0,5M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 6,5, Liquid

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GTE buffer

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

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HEPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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G-Biosciences Acetate Buffer

Supplier: G-Biosciences

Acetate buffer at pH 5,0 and 5,5 are supplied as ready to use solutions that eliminate the calculation, formulation and weighing errors.

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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

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Phosphate, 0,2 M buffer solution, pH 6,8

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 6,8, Liquid

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).