Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

Transformation buffer-2, pH 7,5

Supplier: Thermo Fisher Scientific

Cloning Buffer, Transformation buffer

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TRIS-buffered saline (TBS, 20X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X)

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HEPES, 1,0 M buffer solutions

Supplier: Thermo Fisher Scientific

HEPES buffer solution, 1 mol/L, pH value: 9,0, Liquid

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TRIS-buffered saline (TBS, 10X) pH 7,4

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

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MOPS-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MOPS-SDS running buffer (20X)

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Buffer, potassium phosphate, 0,2 M solution

Supplier: Thermo Fisher Scientific

Potassium phosphate, 0,2M buffer solution, pH 7,0, Liquid

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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100

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pH buffer solutions, referenced at 20 °C, Fluka™

Supplier: Honeywell Chemicals

pH Buffer solution, Glycine, pH value: 13,00, ±0.02, Reference temperature: 20 °C, Fluka™, Plastic bottle, 1×1 L

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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)

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Buffers, MES, 0,2 M solutions

Supplier: Thermo Fisher Scientific

MES, buffer solution, 0,2 M, pH value: 6,0, Colourless, Liquid

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Buffers, PIPES, 1,0 M solutions

Supplier: Thermo Fisher Scientific

Liquid

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Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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MES-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MES-SDS running buffer (20X)

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.