Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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TISAB IV Solution for Determination of Fluoride, Supelco®

Supplier: Merck

Total ionic strength adjustment buffers (TISAB), is generally used for the microdetermination of fluorides.

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ISA (ionic strength adjustment solution: 1 M KCl), Supelco®

Supplier: Merck

Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Standard reference materials and buffer solutions, CertiPUR®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH instruments.

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Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

Sustainable Options Available

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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TISAB solution for Fluoride Determination, Supelco®

Supplier: Merck

TISAB solution for fluoride determination

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Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel, Supelco®

Supplier: Merck

Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel for the determination of sodium, potassium and lithium by flame spectrometry (enth./cont. 50 g/l CsCl + 250 g/l Al(NO₃)₃ * 9 H₂O)

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Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

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EDTA Buffer, pH 8,5, 10X, Antigen Retriever

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.