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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

Cell Meter™ Cell Viability Assay Kit *NIR Fluorescence Optimised for Fluorescence Microplate Reader*

Cell Meter™ Cell Viability Assay Kit *NIR Fluorescence Optimised for Fluorescence Microplate Reader*

Supplier: AAT Bioquest

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

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Amplite® Colorimetric Glycerol Assay Kit

Amplite® Colorimetric Glycerol Assay Kit

Supplier: AAT Bioquest

Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.

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Hydrogen peroxide chemiluminescent detection Kit

Supplier: Enzo Life Sciences

For the quantitative determination of hydrogen peroxide in culture supernatants and cell lysates from any species.

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Screen Quest™ Membrane Potential Assay Kits, Red Fluorescence

Screen Quest™ Membrane Potential Assay Kits, Red Fluorescence

Supplier: AAT Bioquest

Membrane potential is the difference in voltage between the interior and exterior of a cell.

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Amplite® Fluorimetric L-Lactate Assay Kit

Amplite® Fluorimetric L-Lactate Assay Kit

Supplier: AAT Bioquest

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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ROS-ID™ superoxide detection kit

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry.

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IHC enzyme antigen retrieval reagent

Supplier: Enzo Life Sciences

IHC enzyme antigen retrieval reagent is designed to unmask immunoreactive sites altered by fixation and/or the embedding process.

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Flow cytometry fixation/permeabilisation kit

Flow cytometry fixation/permeabilisation kit

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

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Green detection kit, LYSO-ID®

Supplier: Enzo Life Sciences

LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

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Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry*

Supplier: AAT Bioquest

Our Cell Meter™ FITC-Annexin V binding assay kit is designed to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS).

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Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Pierce™ BCA Protein Assay Kits - Reducing Agent Compatible

Supplier: Thermo Fisher Scientific

This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

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Live or Dead™ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*

Live or Dead™ Cell Viability Assay Kit *Red/Blue Dual Fluorescence*

Supplier: AAT Bioquest

This Live or Dead™ Cell Viability Assay Kit uses two fluorescent indicators: Cellbrite™ Red (Ex/Em = 613/631 nm) for labelling viable cells and a cell-impermeable DNA-binding dye Nuclear Blue™ DCS1 (Ex/Em = 360/450 nm) for labelling dead cells with damaged membranes.

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Screen Quest™ Live Cell cAMP Assay Service Pack

Screen Quest™ Live Cell cAMP Assay Service Pack

Supplier: AAT Bioquest

G protein coupled receptors (GPCR) are one of the largest receptor classes targeted by drug discovery programs.

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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

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ROS-ID™ ROS/RNS Detection kit

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

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Screen Quest™ Colorimetric Glucose Uptake Assay Kit

Screen Quest™ Colorimetric Glucose Uptake Assay Kit

Supplier: AAT Bioquest

Glucose transport systems are responsible for transporting glucose across cell membranes.

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Cell Meter™ Autophagy Assay Kit *Green Fluorescence*

Cell Meter™ Autophagy Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

This Cell Meter™ autophagy kit employs autophagy Green™ as a specific autophagosome marker to analyse the activity of autophagy.

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Amplite® Fluorimetric L-Alanine Assay Kit

Amplite® Fluorimetric L-Alanine Assay Kit

Supplier: AAT Bioquest

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Cell Meter™ Fluorimetric Cellular Lipid Peroxidation Assay Kit

Supplier: AAT Bioquest

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

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Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit

Amplite® Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit

Supplier: AAT Bioquest

Nitric oxide (NO) is an important biological regulator involved in numbers of physiological and pathological processes.

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PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV Absorption*

PhosphoWorks™ Colorimetric MESG Phosphate Assay Kit *UV Absorption*

Supplier: AAT Bioquest

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

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Amplite® Colorimetric Acetylcholinesterase Assay Kit

Amplite® Colorimetric Acetylcholinesterase Assay Kit

Supplier: AAT Bioquest

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit, Optimized for Difficult Cell Lines

Screen Quest™ Fluo-8 Medium Removal Calcium Assay Kit, Optimized for Difficult Cell Lines

Supplier: AAT Bioquest

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

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Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*

Cell Meter™ Fixed Cell and Tissue TUNEL Apoptosis Assay Kit *Deep Red Fluorescence*

Supplier: AAT Bioquest

Cell Meter™ TUNEL apoptosis assay kit provides a robust tool for conveniently detecting apoptosis caused by DNA fragmentation.

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Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimised for Flow Cytometry*

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimised for Flow Cytometry*

Supplier: AAT Bioquest

The Cell Meter™ assay kits are a set of tools for monitoring cell viability and proliferation.

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IHC enhancer (DAB, plus), HIGHDEF®

IHC enhancer (DAB, plus), HIGHDEF®

Supplier: Enzo Life Sciences

HIGHDEF® IHC enhancer (DAB, plus) is a DAB signal enhancer used to increase intensity of immunostaining using DAB as chromogen.

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Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Our Amplite® Fluorometric 20S Proteasome Assay Kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.

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CF® Dye Dextran 40000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group.

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Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Cell Meter™ Cellular Senescence Activity Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Cellular Senescence is an irreversible growth arrest triggered in order to prevent growth in DNA damaged cells.

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Cell Meter™ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*

Cell Meter™ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Our Cell Meter™ live cell caspases activity assay kits are based on fluorescent FMK inhibitors of caspases.

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