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You searched for: Assays

Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

Nitric oxide reagents and kits

Nitric oxide reagents and kits

Supplier: Biotium

Nitric oxide donors, nitric oxide synthase inhibitors and nitric oxide detection reagents and kits for nitric oxide (NO) studies.

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Acetyltransferase activity kit

Supplier: ENZO LIFE SCIENCES

For the measurement of acetyl-CoA dependent acetyltransferase activity in purified in vitro samples that do not contain reductants from any species.

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Prostaglandin Screening Library III

Prostaglandin Screening Library III

Supplier: Cayman Chemical

For A and J series prostaglandin analog screening.

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NF[kappa]B transcription factor assay kits

Supplier: Rockland Immunochemicals

These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.

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ER-ID™ green assay kit

Supplier: ENZO LIFE SCIENCES

The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.

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Superoxide dismutase assay kit

Superoxide dismutase assay kit

Supplier: Cayman Chemical

Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

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OXI-TEK TBARS assay kit

OXI-TEK TBARS assay kit

Supplier: ENZO LIFE SCIENCES

The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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APO-BRDU™ kit, TUNEL Assay

APO-BRDU™ kit, TUNEL Assay

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

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β-Hydroxybutyrate (Ketone Body) Assay Kit

β-Hydroxybutyrate (Ketone Body) Assay Kit

Supplier: Cayman Chemical

A sensitive tool for measuring β-HB levels in plasma, serum, or urine.

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LEADING LIGHT® Wnt reporter cell line

Supplier: ENZO LIFE SCIENCES

Wnt reporter cell line for discovering Wnt pathway modulators.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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eFluxx-ID® multidrug resistance assay kit

Supplier: ENZO LIFE SCIENCES

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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Cyclic-AMP fluorescent assay kits, CatchPoint™

Cyclic-AMP fluorescent assay kits, CatchPoint™

Supplier: MOLECULAR DEVICES

CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

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NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: ENZO LIFE SCIENCES

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

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Cyclic ucleotide phosphodiesterase assay kit components

Supplier: ENZO LIFE SCIENCES

3', 5'-cGMP substrate

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Glycolysis Cell-Based Assay Kit

Glycolysis Cell-Based Assay Kit

Supplier: Cayman Chemical

A colorimetric-based method for detecting L-lactate in culture medium.

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Apoptosis and necrosis assay kit, NucView™488 RedDot™2

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

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SOD activity kit

Supplier: ENZO LIFE SCIENCES

ELS offers a large collection of superoxide dismutase products for oxidative stress research.

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Hydrogen peroxide colorimetric detection kit

Supplier: ENZO LIFE SCIENCES

Hydrogen peroxide colorimetric detection kit provides a fast and higher throughput 96-well plate based homogeneous assay for the measurement of hydrogen peroxide in a wide variety of samples.

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Blue IHC chromogen (HRP), HIGHDEF®

Supplier: ENZO LIFE SCIENCES

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

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Calcium assay kits, Fura-2 QBT™ Calcium kit

Calcium assay kits, Fura-2 QBT™ Calcium kit

Supplier: MOLECULAR DEVICES

The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.

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Cell viability assay kits

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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Fluorescent calcium indicator, GFP-CERTIFIED® FLUOFORTE®

Supplier: ENZO LIFE SCIENCES

GFP-CERTIFIED® FLUOFORTE® is a fluorescent calcium indicator relative to other red fluorescent dyes.

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Catalase fluorometric detection kit

Supplier: ENZO LIFE SCIENCES

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.

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CV-Cathepsin K detection kit

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin K LeuArg peptide recognition sequence, this kit allows the detection of cathepsin K activity in living cells by fluorescence microscopy.

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DNMT colorimetric drug discovery tests

DNMT colorimetric drug discovery tests

Supplier: ENZO LIFE SCIENCES

DNMT colorimetric drug discovery kit is a simple method to detect DNA methylation activity or inhibition.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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