DAF-2 diacetate

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DAF-2 diacetate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cell permeable fluorescent detector of nitric oxide in living cells. 4,5-Diaminofluorescein diacetate (DAF-2 DA) has been used as a fluorescent dye for NO (nitric oxide) detection.

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DAF-2 diacetate

Supplier: Abcam

Cell-permeable fluorescent nitric oxide (NO) indicator. Deacetylated by intracellular esterases to DAF-2. DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T).

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DAF-2 diacetate 5 mM in DMSO ≥98% (by HPLC)

Supplier: Enzo Life Sciences

Fluorescent probe for NO detection. Cell permeable.

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DAF-2 diacetate

Supplier: Abcam

Fluorescent nitric oxide probe; membrane-permeable. Once inside cell it reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein DAF-2T. Determines nitric oxide synthase activity. Has a 2 to 5 nM detection limit at neutral pH. Also available as a solid.

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DAF-2 diacetate for nitric oxide detection

DAF-2 diacetate for nitric oxide detection

Supplier: AAT Bioquest

DAF-2 diacetate is a more cell-permeable version of DAF-2, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO).

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VWR®, Nitrile Gloves

#: 112-2373

: VWR Collection

VWR®, Transfer Pipettes, High Performance

#: 612-1681

: VWR Collection

Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

#: 83639.320

: VWR Chemicals

Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

#: 20864.320

: VWR Chemicals

VWR® Serological Pipette, Standard Line

#: 612-3700

: VWR Collection

VWR® NITRILE LIGHT, Nitrile Gloves

#: 112-2756

: VWR Collection

VWR® Facial Tissues

#: 115-0600

: VWR Collection

Sealing film for manual application, Parafilm® M

#: 291-0057

: HEATHROW

Nitrile gloves, Kimtech™ Purple Nitrile™

#: 112-4847

: Ansell

VWR®, Laboratory Bottles, Narrow Neck, with Screw Cap

#: 215-1592

: VWR Collection

Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

#: 83640.320

: VWR Chemicals

Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

#: 31770.294

: VWR Chemicals

pH indicator strips, MQuant®, Supelco®

#: 1.09535.0001

: Merck

Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

#: 1.00030.2500

: Merck

Single-use syringes, 2-piece, HENKE-JECT®

#: 613-2001

: HENKE SASS WOLF

VWR® AVS TITRINORM Buffer Solutions, 20 °C

#: 32096.291

: VWR Chemicals

Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

#: 83645.320

: VWR Chemicals

2-Propanol ≥99.8%, HiPerSolv CHROMANORM® for HPLC

#: 20880.320

: VWR Chemicals