Loading Buffers

The electrophoresis buffers maintain consistent pH levels during DNA, RNA, and other protein separation. Providing ions that transfer the applied current, the low charge solutions facilitate different molecular sizes to travel at various rates for analyses. Offering additional protection to proteins, the preparative substances prevent degradation for guaranteed sample viability and accurate testing. Available in various molecular weights, the best electrophoresis buffer can be selected based on the specific macromolecules being handled.

Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

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Laemmli SDS sample buffer, reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, reducing, 4X

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LDS-sample buffer (4X), non-reducing

Supplier: Thermo Fisher Scientific

LDS-sample buffer (4X), non-reducing is used in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

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IEF Sample buffer (4X) pH 3 to 7 with lysine

Supplier: Thermo Fisher Scientific

Loading Buffer, IEF Sample Buffer (4X) with Lysine

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Low pH SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

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Low pH Phosphate SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS phosphate sample buffer, 140 ml (40 mM Phosphate, pH 6,5, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a China Pharmacopeia compliant buffer.

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SDS Protein Gel Loading Solution, 2X

Supplier: MARKET SOURCE PART PROCESS

SDS Protein Gel Loading Solution, 2X is formulated at the traditional 2X stocks, for standard volume of sample to be loaded per well. Simply load the gel sample wells and visualise progress of the electrophoresis run by monitoring the location of the blue dye front.