You searched for: Materiales educativos de biología

Supplier: G-Biosciences

The physical properties of proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.

Supplier: G-Biosciences

This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

Supplier: G-Biosciences

Dot blotting is a simple technique to identify a known protein in a biological sample. The ease and simplicity of the technique makes dot blotting an ideal diagnostic tool.

Supplier: EDVOTEK

Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.

Supplier: EDVOTEK

In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members.

Supplier: EDVOTEK

DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis.

Supplier: EDVOTEK

The three most frequently used restriction enzymes are Eco RI, Bam HI and Hind III. Each enzyme catalyses cleavage at the defined base sequence. All enzymes are lyophilised and contain 1500 units. One unit is defined as the amount of enzyme required to digest 1,0 µg of lambda DNA in 60 minutes at 37 °C in a total reaction mixture of 50 µl.

Supplier: EDVOTEK

This experiment helps teach students about restriction enzyme digests in the context of forensic science. Students cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.

Supplier: EDVOTEK

Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.

Supplier: EDVOTEK

This simple experiment demonstrates detection of the mutation that causes Sickle Cell Anaemia. In this simulation, students use electrophoresis to separate dyes that represent patient samples and controls.

Supplier: EDVOTEK

Coronary heart disease and stroke are major causes of death in the Western world. Elevated blood cholesterol levels are a serious risk factor in both conditions. The genetic disease familial hypersholesterolemia (FH) causes an increase in blood levels of the bad form of cholesterol, low density lipoprotein (LDL). In untreated patients with the mutant FH gene, the condition can cause premature death. This experiment introduces the colorimetric enzymatic reaction which is the basis of the clinical cholesterol test. In addition, using agarose gel electrophoresis, students can analyse a simulated genetic screening for a disease.

Supplier: EDVOTEK

Ready-to-Load™ kit to show how electrophoresis separates molecules on the basis of size and charge.

Supplier: EDVOTEK

In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

Supplier: EDVOTEK

This kit is for teaching students how people's physical traits are a reflection of their genes. In this simulation, students use electrophoresis to separate dyes which represent genetic traits.

Supplier: EDVOTEK

Learn the basics of DNA sequencing and sequence homology with this lab activity.

Supplier: EDVOTEK

Genetic testing can be used to identify people with a genetic condition which caused them to have an elevated level of cholesterol and which can be fatal. Students can see how genetic testing is carried out and learn about DNA electrophoresis.

Supplier: EDVOTEK

This Ready-to-Load™ kit shows how a restriction enzyme can be used to screen DNA for Sickle Cell Anaemia.

Supplier: EDVOTEK

For learning about the diversity of proteins by studying the electrophoretic profiles of various sources. Students separate proteins from bacterial, plant, serum, and milk proteins alongside a standard protein marker.

Supplier: EDVOTEK

Kit allows students to construct DNA models and then extract DNA from onions, strawberries or bananas.

Supplier: EDVOTEK

Experiment using fluorescence and electrophoresis to solve the mystery of the invisible bands.

Supplier: EDVOTEK

Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

Supplier: EDVOTEK

Small-scale, rapid isolation of plasmid DNA is a routine procedure used for screening and analysis of recombinant DNAs in cloning and sub-cloning experiments. In this experiment, students isolate plasmid DNA without the use of toxic chemicals such as phenol or chloroform.

Supplier: EDVOTEK

Solve the mystery of two boys separated from their parents a decade ago. Their biological mother is identified by mitochondrial DNA and their biological father from chromosomal DNA.

Supplier: EDVOTEK

El ADN del bacteriófago Lambda es una molécula lineal bien caracterizada que contiene seis sitios de reconocimiento para Eco RI (5 sitios distintos; 2 de ellos de tamaño muy similar). En este experimento, el ADN de Lambda es digerido por la endonucleasa Eco RI. Los productos de la digestión se analizan mediante electroforesis en gel de agarosa.

Supplier: EDVOTEK

This easy and safe experiment allows students to learn about enzyme catalysis, the nature of enzyme action, and protein structure-function relationship. Students will perform an enzyme assay and determine the rate of the enzymatic reaction.

Supplier: EDVOTEK

This experiment introduces a simple method to determine protein subunit molecular weights using horizontal electrophoresis. As the protein standards and 'unknowns' are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is protein-grade agarose, which provides an alternative to the use of polyacrylamide gels.

Supplier: EDVOTEK

Microarray technology allows scientists to screen large numbers of samples in one assay. This technology allows high-throughput screening of small samples and allows comparisons to be made between healthy and unhealthy tissues. In this experiment, students apply simulated DNA and RNA samples to a membrane to screen for positive and negative samples.

Supplier: EDVOTEK

Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.

Supplier: EDVOTEK

Bovine spongiform encephalophathy (BSE), also known as Mad Cow Disease, is a neurodegenerative, fatal condition in cattle. Consuming BSE-infected beef is believed to be the cause of a similar condition in humans, Creutzfeldt-Jakob disease. In this experiment, students examine simulated PCR products from several feed mills, to determine any possible violations of the ban on including animal parts in cattle feed.

Supplier: EDVOTEK

Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from E. coli. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.