"Stemcell Technologies"

Anti-Igm Isotype Control Mouse Monoclonal Antibody [clone: MM-30] (Alexa Fluor® 488)

Supplier: Stemcell Technologies

Mouse monoclonal IgM, kappa isotype control antibody, Alexa Fluor® 488conjugated.

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Dispase (1 U/ml)

Supplier: Stemcell Technologies

1 U/ml dispase in DMEM/F-12.

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Human Recombinant ASAM, His tag

Supplier: Stemcell Technologies

Adipocyte-specific adhesion molecule (ASAM) may play a role in cell-cell adhesion as it is expressed in epithelial cells of various tissues and is associated with tight junctions (Raschberger et al., Sze et al.). This type 1 transmembrane protein belongs to the cortical thymocyte marker in the Xenopus (CTX) family within the immunoglobulin superfamily. Sequence alignment analysis has shown that ASAM shares conserved characteristics with other CTX-like proteins, including extracellular V-type and C2-type domains, a transmembrane region, and a cytoplasmic tail (Raschperger et al.). ASAM is also expressed in adipocytes, and was found to be upregulated in white adipose tissue (WAT) in a rodent model of obesity and type II diabetes (Eguchi et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, adipocyte-specific adhesion molecule from STEMCELL comes lyophilised with ≥94% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.

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ArciTect™ Cas9-eGFP Nuclease

Supplier: Stemcell Technologies

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing.

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Anti-CD44 Rat Monoclonal Antibody [clone: IM7] (PE)

Supplier: Stemcell Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD44 (tissue non-specific alkaline phosphatase), PE-conjugated.

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Human Recombinant IL-11

Supplier: Stemcell Technologies

Interleukin 11 (IL-11) is a pleiotropic cytokine with effects on various tissues including the bone marrow, brain, and intestinal mucosa (Du andamp; Williams). It belongs to the IL-6 family of cytokines that share a common signal transducer, gp130. IL-11 induces the proliferation of hematopoietic stem cells (Lemoli et al.) and megakaryocytic progenitor cells (Bruno et al.), the maturation of megakaryocytes (Burstein et al.), and the production of platelets (Neben et al.). IL-11 is produced by a variety of cell types including hematopoietic cells, mesenchymal cells, epithelial cells, and neuronal cells. It was first cloned from a cDNA library of the human bone marrow-derived stromal cell line KM-102 (Kawashima et al.). The binding of IL-11 to its receptor induces heterodimerization with the gp130 subunit and activation of JAK tyrosine kinases. IL-11 was the first pharmacologic agent approved for the treatment of chemotherapy-induced thrombocytopenia. IL-11 also plays a role in cancer progression by inducing the proliferation of epithelial cancer cells and the survival of metastatic cells at distant organs. Recently, IL-11 has gained interest for its role in the pathogenesis of diseases in dysregulated mucosal homeostasis associated with STAT3 upregulation, including gastrointestinal cancers (Putoczki et al.).