"Stemcell Technologies"

Supplier: Stemcell Technologies

Hydrocortisone stock solution (200X) for use with PneumaCult™-ALI, MammoCult™, EpiCult™.

Supplier: Stemcell Technologies

Pre-aliquoted methylcellulose-based medium with recombinant cytokines. 24×3 ml vials.

Supplier: Stemcell Technologies

For detection and measurement of human interferon-gamma.

Supplier: Stemcell Technologies

Purified tropoelastin, human, lyophilised, for tissue engineering research and cell culture - 1 mg

Supplier: Stemcell Technologies

Fibroblast growth factor 10 (FGF-10) is a member of the fibroblast growth factor (FGF) family predominantly expressed by mesenchymal fibroblasts during embryonic development (Emoto et al.; Igarashi et al.). It binds with high affinity to fibroblast growth factor receptor 2-IIIb (FGFR2-IIIb), and also has a weaker affinity for FGFR1-IIIb (Beer et al.). FGF-10 and FGF-7 have similar receptor binding properties and target cell specificities but are differentially regulated by components of the extracellular matrix (Emoto et al.; Igarashi et al.). FGF-10 has been shown to mediate epithelial-mesenchymal interactions, which are essential to lung development (Sekine et al; Ware and Matthay). FGF-10 also has a role in mobilisation and proliferation of lung-resident mesenchymal stem cells (MSCs) and protection and repair against acute lung injury (Tong et al.; Ware and Matthay), as well as endodermal differentiation of human pluripotent stem cells to insulin-producing pancreatic-like cells (Takeuchi et al.).

Supplier: Stemcell Technologies

15 ml centrifuge tube with an insert for density gradient centrifugation

Supplier: Stemcell Technologies

Mouse monoclonal antibody against human TNF-alpha, biotin-conjugated.

Supplier: Stemcell Technologies

Immunomagnetic isolation of untouched human CD8+ T cells.

Supplier: Stemcell Technologies

Fibroblast growth factor 7 (FGF-7) is a member of the FGF family, and acts exclusively through a subset of FGF receptor isoforms expressed predominantly by epithelial cells (Finch and Rubin). FGF-7 seems to act specifically on epithelial cells and stimulates proliferation, migration, and differentiation of these cells, and also participates in epithelial protection and repair both in vitro and in vivo (Finch and Rubin; Werner). In contrast, FGF-7 is produced solely by cells of mesenchymal origin, and functions as a paracrine mediator of mesenchymal-epithelial communication (Rubin et al.). FGF-7 has also been shown to supplement several wound-healing properties of bioengineered skin (Erdag et al.) and to induce autophagy in human keratinocytes (Belleudi et al.). Additionally, FGF-7 has a role in the differentiation of pluripotent stem cell to endodermal pancreatic-like insulin-producing cells and thymic epithelial cells (Inami et al.; Niu et al.). This product is animal component-free.

Supplier: Stemcell Technologies

Animal component-free and protein-free cryopreservation formulation.

Supplier: Stemcell Technologies

Human T cell activation and expansion reagent. 5×2 ml vials.

Supplier: Stemcell Technologies

Mouse monoclonal IgG1, kappa isotype control antibody, Alexa Fluor® 488-conjugated.

Supplier: Stemcell Technologies

Mouse monoclonal IgM, kappa isotype control antibody, Alexa Fluor® 488­conjugated.

Supplier: Stemcell Technologies

1 U/ml dispase in DMEM/F-12.

Supplier: Stemcell Technologies

Adipocyte-specific adhesion molecule (ASAM) may play a role in cell-cell adhesion as it is expressed in epithelial cells of various tissues and is associated with tight junctions (Raschberger et al., Sze et al.). This type 1 transmembrane protein belongs to the cortical thymocyte marker in the Xenopus (CTX) family within the immunoglobulin superfamily. Sequence alignment analysis has shown that ASAM shares conserved characteristics with other CTX-like proteins, including extracellular V-type and C2-type domains, a transmembrane region, and a cytoplasmic tail (Raschperger et al.). ASAM is also expressed in adipocytes, and was found to be upregulated in white adipose tissue (WAT) in a rodent model of obesity and type II diabetes (Eguchi et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, adipocyte-specific adhesion molecule from STEMCELL comes lyophilised with ≥94% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.

Supplier: Stemcell Technologies

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing.