Supplier: STEMCELL Technologies

RANTES (regulated upon activation, normal T cell expressed and secreted), also known as CCL5, is a member of the CC family of chemokines and is able to recruit leukocytes to sites of inflammation (Schall et al.). RANTES is secreted by T lymphocytes, macrophages, platelets, synovial fibroblasts, tubular epithelium, and certain types of tumor cells (Aldinucci and Colombatti; Soria and Ben-Baruch). This chemokine exerts its effect by interacting with the chemokine receptors CCR1, CCR3, CCR4, and CCR5. RANTES plays an active role in recruiting a variety of leukocytes into inflammatory sites, including T cells, macrophages, eosinophils, and basophils. In collaboration with certain cytokines that are released by T cells such as IL-2 and IFN-γ, RANTES also induces the activation and proliferation of NK cells to generate CC chemokine-activated killer cells, which are highly cytolytic (Lv et al.; Maghazachi et al.). It has been shown that RANTES produced by CD8+ T cells inhibits HIV infection of primary human peripheral blood mononuclear cells (Appay and Rowland-Jones; Cocchi et al.).

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG2 antibody against mouse T cell receptor gamma/delta, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD4, PE-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b, kappa isotype control antibody, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse, toad CD8a, FITC-conjugated.

Supplier: STEMCELL Technologies

Epidermal growth factor (EGF) is characterized by high affinity binding to various EGF receptors (EGFRs) and the production of mitogenic responses (Carpenter and Cohen). EGF promotes EGFR dimerization, resulting in activation of downstream pathways including PI3K, ERK1/2, JAK/STAT, β-catenin, and calcium signaling. EGF is secreted by the gut-associated salivary and Brunner’s glands, is found in a variety of body fluids, and stimulates cell proliferation and differentiation in rodent and neonatal human intestine (Wright et al.). Central nervous system stem cells also proliferate in response to the EGF stimulus (Reynolds and Weiss).

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Insulin-like growth factor 1 (IGF-I) is a polypeptide that belongs to the family of insulin-like growth factors that are similar in molecular structure to proinsulin. IGF-I binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway and also activates ERK1/2 signaling. IGF-I is required for embryonic development, and it is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells (Wang et al.). Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and has been shown to regulate lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs (Heemskerk et al.).

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Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220).

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b, biotin-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG antibody against mouse CD11c, FITC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD45, unconjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG1 antibody against mouse CD3e, FITC-conjugated.

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Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin supports maintenance of undifferentiated human embryonic stem cells in vitro, and can be used to prevent spontaneous differentiation in the short term (Chaturvedi et al.). Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al.), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al.).

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Leukemia inhibitory factor (LIF) is an interleukin 6 class cytokine that regulates a broad variety of developmental functions. After LIF binds to the LIF receptor (LIFR), LIFR associates with gp130 and activates JAK/STAT and MAPK signaling (Auernhammer and Melmed; Suman et al.). LIFR activation of STAT3 is essential for maintaining the mouse embryonic stem cell phenotype (Niwa et al.). Produced by the endometrium, LIF plays an important autocrine and paracrine role in implantation by regulating proliferation, invasion, and differentiation of trophoblasts following blastocyst attachment (Auernhammer and Melmed; Suman et al.). Human LIF can be used for the maintenance of mouse embryonic stem cells; however, mouse LIF cannot bind to the human receptor, thus rendering mouse LIF inactive (Dahéron et al.). LIF is produced by CD4+ and activated regulatory T cells, and promotes Foxp3 expression, while repressing Th17 lineage-specific genes (Metcalfe). LIF is also secreted by mesenchymal stromal cells, where it supports hematopoiesis and immune modulation (Nasef et al.).

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Mouse monoclonal IgG1 antibody against human, rat CD36, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E), biotin-conjugated.

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Mouse monoclonal IgG1 antibody against rhesus red blood cells, unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human epithelial cells, unconjugated.

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Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD184 (CXCR4), PE-conjugated.

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CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE.

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Medium for the culture and differentiation of human monocytes into dendritic cells and their maturation.

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Serum-free culture supplement for expansion of human megakaryocytes.

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Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD83, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD117 (c-Kit), APC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4, PE-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4, biotin-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG antibody against mouse CD11c, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human CD235ab (glycophorin A/B), unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b, APC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2b antibody against mouse CD16/CD32, FITC-conjugated.